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LearnNB600-261
antibody from Novus Biologicals
Targeting: BCL11A
BCL11A-L, BCL11A-S, BCL11A-XL, CTIP1, EVI9, HBFQTL5, ZNF856
Western blot
Flow cytometryAntibody data
- Antibody Data
- Antigen structure
- References [6]
- Comments [0]
- Validations
- Western blot [3]
- Immunoprecipitation [2]
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Validation data
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- Product number
- NB600-261 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB600-261, RRID:AB_10001833
- Product name
- Rabbit Polyclonal Ctip1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. NB600-261 is specific for human Bcl-11a protein.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ul
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C. Do not freeze.
Submitted references The Coup-TFII orphan nuclear receptor is an activator of the γ-globin gene.
A natural regulatory mutation in the proximal promoter elevates fetal globin expression by creating a de novo GATA1 site.
Natural regulatory mutations elevate the fetal globin gene via disruption of BCL11A or ZBTB7A binding.
Hydroxyurea differentially modulates activator and repressors of γ-globin gene in erythroblasts of responsive and non-responsive patients with sickle cell disease in correlation with Index of Hydroxyurea Responsiveness.
Bcl11a controls Flt3 expression in early hematopoietic progenitors and is required for pDC development in vivo.
Transcriptional silencing of {gamma}-globin by BCL11A involves long-range interactions and cooperation with SOX6.
Fugazza C, Barbarani G, Elangovan S, Marini MG, Giolitto S, Font-Monclus I, Marongiu MF, Manunza L, Strouboulis J, Cantù C, Gasparri F, Barabino SML, Nakamura Y, Ottolenghi S, Moi P, Ronchi AE
Haematologica 2020 Feb 27;
Haematologica 2020 Feb 27;
A natural regulatory mutation in the proximal promoter elevates fetal globin expression by creating a de novo GATA1 site.
Martyn GE, Wienert B, Kurita R, Nakamura Y, Quinlan KGR, Crossley M
Blood 2019 Feb 21;133(8):852-856
Blood 2019 Feb 21;133(8):852-856
Natural regulatory mutations elevate the fetal globin gene via disruption of BCL11A or ZBTB7A binding.
Martyn GE, Wienert B, Yang L, Shah M, Norton LJ, Burdach J, Kurita R, Nakamura Y, Pearson RCM, Funnell APW, Quinlan KGR, Crossley M
Nature genetics 2018 Apr;50(4):498-503
Nature genetics 2018 Apr;50(4):498-503
Hydroxyurea differentially modulates activator and repressors of γ-globin gene in erythroblasts of responsive and non-responsive patients with sickle cell disease in correlation with Index of Hydroxyurea Responsiveness.
Zhu X, Hu T, Ho MH, Wang Y, Yu M, Patel N, Pi W, Choi JH, Xu H, Ganapathy V, Kutlar F, Kutlar A, Tuan D
Haematologica 2017 Dec;102(12):1995-2004
Haematologica 2017 Dec;102(12):1995-2004
Bcl11a controls Flt3 expression in early hematopoietic progenitors and is required for pDC development in vivo.
Wu X, Satpathy AT, Kc W, Liu P, Murphy TL, Murphy KM
PloS one 2013;8(5):e64800
PloS one 2013;8(5):e64800
Transcriptional silencing of {gamma}-globin by BCL11A involves long-range interactions and cooperation with SOX6.
Xu J, Sankaran VG, Ni M, Menne TF, Puram RV, Kim W, Orkin SH
Genes & development 2010 Apr 15;24(8):783-98
Genes & development 2010 Apr 15;24(8):783-98
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Ctip1 Antibody [NB600-261] - Detection of Mouse Bcl11a by Western Blot. Samples: Whole cell lysate (50 ug) from L1210, TCMK-1, and 4T1 cells. Antibodies: Affinity purified rabbit anti-Bcl11a antibody NB600-261 used for WB at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Ctip1 Antibody [NB600-261] - Detection of human Ctip1 (Bcl11a) by western blot. Samples: Whole cell lysate from Ramos (15 and 50 ug), HEK293T (T; 50 ug) and Jurkat (J; 50 ug) cells. Antibodies: Affinity purified rabbit anti-Ctip1 antibody NB600-261 used at 1 ug/ml. Detection: Chemiluminescence with exposure times of 30 seconds.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Ctip1 Antibody [NB600-261] - Whole cell lysate (50 ug) from L1210, TCMK-1, and 4T1 cells. Antibodies: Affinity purified rabbit anti-Bcl11a antibody used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: Ctip1 Antibody [NB600-261] - Detection of Human Bcl11a on HeLa whole cell lysate using NB600-261. Bcl11a was also immunoprecipitated using affinity purified rabbit anti-Bcl11a antibodies NB600-258 and NB600-259.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: Ctip1 Antibody [NB600-261] - Detection of human Ctip1 (Bcl11a) by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from Ramos cells. Antibodies: Affinity purified rabbit anti-Ctip1 antibody NB600-261 used for IP at 6 ul/mg lysate. Ctip1 was also immunoprecipitated by a rabbit anti-Ctip1 antibodies (NB600-258, NB600-259) and rabbit anti-Ctip1 recombinant monoclonal antibody [BLR073G] (NBP2-78710). For blotting immunoprecipitated Ctip1, NBP2-78710 was used at 1:1000. Chemiluminescence with an exposure time of 75 seconds.
