MA5-29052

antibody from Invitrogen Antibodies

Targeting: BLVRB FLR, SDR43U1

Western blot (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-29052

Provider

Invitrogen Antibodies

Product name

Flavin reductase Recombinant Rabbit Monoclonal Antibody (9)

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Description

This product is preservative free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%-0.1%).

Antibody clone number

9

Concentration

1 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of Flavin reductase in Lane A: Hela Whole Cell Lysate (30 µg), Lane B: HepG2 Whole Cell Lysate (30 µg), Lane C: K562 Whole Cell Lysate (30 µg). Samples were probed using a Flavin reductase Monoclonal Antibody (Product # MA5-29052) at a 1:500 dilution, followed by a Goat Anti-Rabbit IgG (H+L), Dylight 800 Secondary Antibody at a 1:10000 dilution. Western blot was performed under reducing conditions. Predicted band size:22 kDa. Observed band size:22 kDa.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using Flavin reductase Recombinant Rabbit Monoclonal Antibody (9) (Product # MA5-29052) at 1:500 dilution. Lane A: Hela Whole Cell Lysate, Lane B: HepG2 Whole Cell Lysate, Lane C: K562 Whole Cell Lysate. Lysates/proteins at 30 μg per lane. Secondary Goat Anti-Rabbit IgG H&L (DyLight™ 800) at 1:10,000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 22 kDa. Observed band size: 22 kDa.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flavin reductase Immunoprecipitation using: Lane A: 0.5 mg Hela Whole Cell Lysate, Lane B: 0.5 mg HepG2 Whole Cell Lysate, Lane C: 0.5 mg K562 Whole Cell Lysate, Lane D: 0.5 mg Caco-2 Whole Cell Lysate 2 µL with Flavin reductase Recombinant Rabbit Monoclonal Antibody (9) (Product # MA5-29052) and 15 µL of 50 % Protein G agarose. Primary antibody: Flavin reductase Recombinant Rabbit Monoclonal Antibody (9), at 1:100 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5,000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 22 kDa. Observed band size: 22 kDa.