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Western blot
Flow cytometryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
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- Product number
- MA5-25957 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ACSF2 Monoclonal Antibody (OTI6E4)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- OTI6E4
- Vial size
- 100 µL
- Concentration
- 0.53 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of ACSF2 in the following cell types: HepG2, HeLa, SVT2, A549, COS7, Jurkat, MDCK, PC12, MCF7. The samples were incubated with monoclonal ACSF2 (Product # MA5-25957) using 35 µg of sample and an antibody dilution of 1:200.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of ACSF2 in COS7 cells. Cells were transfected with a plasmid overexpressing ACSF2 and probed with a ACSF2 monoclonal antibody (Product # MA5-25957).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry was performed on paraffin-embedded carcinoma of human thyroid tissue. To expose target proteins, 10mM citric buffer, pH6.0, 100°C for 10min was used. Following antigen retrieval, tissues were probed with a ACSF2 monoclonal antibody (Product # MA5-25957).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry was performed on paraffin-embedded carcinoma of human lung tissue. To expose target proteins, 10mM citric buffer, pH6.0, 100°C for 10min was used. Following antigen retrieval, tissues were probed with a ACSF2 monoclonal antibody (Product # MA5-25957).
