MA1-20893

antibody from Invitrogen Antibodies

Targeting: GYPA CD235a, GPA, MN, MNS

Immunohistochemistry (Data presented on provider website)

Flow cytometry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

MA1-20893

Provider

Invitrogen Antibodies

Product name

CD235a Monoclonal Antibody (HIR2)

Antibody type

Monoclonal

Antigen

Synthetic peptide

Description

MA1-20893 detects Glycophorin A in Human samples.

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

HIR2

Vial size

100 µg

Concentration

1 mg/mL

Storage

4° C, do not freeze

References

Submitted references

Complement receptor 3 mediates both sinking phagocytosis and phagocytic cup formation via distinct mechanisms.

Walbaum S, Ambrosy B, Schütz P, Bachg AC, Horsthemke M, Leusen JHW, Mócsai A, Hanley PJ
The Journal of biological chemistry 2021 Jan-Jun;296:100256

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LECT2 drives haematopoietic stem cell expansion and mobilization via regulating the macrophages and osteolineage cells.

Lu XJ, Chen Q, Rong YJ, Yang GJ, Li CH, Xu NY, Yu CH, Wang HY, Zhang S, Shi YH, Chen J
Nature communications 2016 Sep 6;7:12719

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Definitive Hematopoietic Multipotent Progenitor Cells Are Transiently Generated From Hemogenic Endothelial Cells in Human Pluripotent Stem Cells.

Bai H, Liu Y, Xie Y, Hoyle DL, Brodsky RA, Cheng L, Cheng T, Wang ZZ
Journal of cellular physiology 2016 May;231(5):1065-76

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Differential IL-7 responses in developing human thymocytes.

Marino JH, Tan C, Taylor AA, Bentley C, Van De Wiele CJ, Ranne R, Paliotta M, Broughan TA, Teague TK
Human immunology 2010 Apr;71(4):329-33

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry analysis analysis of human peripheral blood using CD235a Monoclonal Antibody (HIR2) (Product # MA1-20893).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 1 Phagocytic cup formation mediated by Fcgamma receptors. A , 3D time-lapse images obtained by spinning disk confocal microscopy showing a WT macrophage (Mph) ingesting a human red blood cell (hRBC) opsonized with mouse IgG antibodies (mouse anti-human CD235a monoclonal IgG2b antibodies). The macrophage was labeled green fluorescent with Alexa Fluor 488-conjugated anti-F4/80 antibodies, and hRBCs were labeled red fluorescent with the plasma membrane stain CellMask Orange. Scale bars, 10 mum. B , ingestion of an IgG-opsonized hRBC by a morphologically spherical macrophage. Note that the tip of the ingested and squeezed hRBC is pinched off during cup closure ( white arrow ). This may serve as an immune defense strategy to lyse ingested cells. Scale bar, 10 mum (grid spacing [3D view] = 3.05 mum). C , lack of ingestion of IgG-opsonized hRBCs by No ITAM (NOTAM) (grid spacing = 6.46 mum) and Fcer1g -/- macrophages (grid spacing = 6.49 mum). NOTAM macrophages contain nonsignaling immunoreceptor tyrosine-based activation motifs (ITAMs) in the Fc receptor gamma chain. D , cumulative plots of phagocytic (ingestion) events for WT, NOTAM, and Fcer1g -/- macrophages. The plots for NOTAM and Fcer1g -/- macrophages are overlaid. The x -axes show individual macrophages. E , summary box plots of phagocytic efficiency, defined as the percentage of IgG-opsonized hRBCs (in contact with a macrophage) which were fully ingested. Medians are represented by a horizontal line with a lilac-colo