14-9987-80

antibody from Invitrogen Antibodies

Targeting: GYPA CD235a, GPA, MN, MNS

Flow cytometry (Recommended by provider)

Antibody Data

Product number

14-9987-80

Provider

Invitrogen Antibodies

Product name

Anti-CD235a (Glycophorin A) Monoclonal Antibody (HIR2 (GA-R2)), eBioscience™

Antibody type

Monoclonal

Antigen

Other

Description

Description: The HIR2 monoclonal antibody reacts with human glycophorin A, a sialoglycoprotein expressed by erythroid precursors and mature circulating red cells. Applications Reported: HIR2 can be used in flow cytometric analysis. Applications Tested: The HIR2 (also GA-R2) antibody has been tested by flow cytometric analysis of normal human peripheral blood cells and cell agglutination assay and can be used at approximately 0.1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 µm post-manufacturing filtered.

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

HIR2 (GA-R2)

Vial size

25 µg

Concentration

0.5 mg/mL

Storage

4° C

References

Submitted references

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Mitchell AJ, Gray WD, Hayek SS, Ko YA, Thomas S, Rooney K, Awad M, Roback JD, Quyyumi A, Searles CD
Scientific reports 2016 Sep 13;6:32651

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Genetic Variability Overrides the Impact of Parental Cell Type and Determines iPSC Differentiation Potential.

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Stem cell reports 2016 Feb 9;6(2):200-12

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PPAR-α and glucocorticoid receptor synergize to promote erythroid progenitor self-renewal.

Lee HY, Gao X, Barrasa MI, Li H, Elmes RR, Peters LL, Lodish HF
Nature 2015 Jun 25;522(7557):474-7

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Mapping the homodimer interface of an optimized, artificial, transmembrane protein activator of the human erythropoietin receptor.

Cohen EB, Jun SJ, Bears Z, Barrera FN, Alonso M, Engelman DM, DiMaio D
PloS one 2014;9(4):e95593

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Altered translation of GATA1 in Diamond-Blackfan anemia.

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Nature medicine 2014 Jul;20(7):748-53

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Prognostic significance of CD44 variant 2 upregulation in colorectal cancer.

Ozawa M, Ichikawa Y, Zheng YW, Oshima T, Miyata H, Nakazawa K, Guan HB, Shiozawa M, Akaike M, Watanabe K, Ota M, Fujii S, Kunisaki C, Ishikawa T, Tanaka K, Akiyama H, Endo I, Taniguchi H
British journal of cancer 2014 Jul 15;111(2):365-74

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EDAG positively regulates erythroid differentiation and modifies GATA1 acetylation through recruiting p300.

Zheng WW, Dong XM, Yin RH, Xu FF, Ning HM, Zhang MJ, Xu CW, Yang Y, Ding YL, Wang ZD, Zhao WB, Tang LJ, Chen H, Wang XH, Zhan YQ, Yu M, Ge CH, Li CY, Yang XM
Stem cells (Dayton, Ohio) 2014 Aug;32(8):2278-89

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A feedback loop consisting of microRNA 23a/27a and the β-like globin suppressors KLF3 and SP1 regulates globin gene expression.

Ma Y, Wang B, Jiang F, Wang D, Liu H, Yan Y, Dong H, Wang F, Gong B, Zhu Y, Dong L, Yin H, Zhang Z, Zhao H, Wu Z, Zhang J, Zhou J, Yu J
Molecular and cellular biology 2013 Oct;33(20):3994-4007

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Terminal differentiation and loss of tumorigenicity of human cancers via pluripotency-based reprogramming.

Zhang X, Cruz FD, Terry M, Remotti F, Matushansky I
Oncogene 2013 May 2;32(18):2249-60, 2260.e1-21

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α-1,6-Fucosyltransferase (FUT8) inhibits hemoglobin production during differentiation of murine and K562 human erythroleukemia cells.

Sasaki H, Toda T, Furukawa T, Mawatari Y, Takaesu R, Shimizu M, Wada R, Kato D, Utsugi T, Ohtsu M, Murakami Y
The Journal of biological chemistry 2013 Jun 7;288(23):16839-47

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Role of ZBP-89 in human globin gene regulation and erythroid differentiation.

Woo AJ, Kim J, Xu J, Huang H, Cantor AB
Blood 2011 Sep 29;118(13):3684-93

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Generation of bivalent chromatin domains during cell fate decisions.

De Gobbi M, Garrick D, Lynch M, Vernimmen D, Hughes JR, Goardon N, Luc S, Lower KM, Sloane-Stanley JA, Pina C, Soneji S, Renella R, Enver T, Taylor S, Jacobsen SE, Vyas P, Gibbons RJ, Higgs DR
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