48-9884-42

antibody from Invitrogen Antibodies
Targeting: GYPA CD235a, GPA, MN, MNS
Provider product page for 48-9884-42

Antibody data

Product number
48-9884-42 - Provider product page
Provider
Invitrogen Antibodies
Product name
CD235a (Glycophorin A) Monoclonal Antibody (6A7M), eFluor™ 450, eBioscience™
Antibody type
Monoclonal
Antigen
Other
Description
Description: The monoclonal antibody 6A7M recognizes human glycophorin A (also known as CD235a). The antibody reacts with the M allele. Glycophorin A is a 151 amino acid sialoglycoprotein found on the erythrocyte (RBC) and erthyroid progenitor cell membrane at about 500,000 copies per cell. The gene for glycophorin resides on chromosome 4 and has 2 allelic forms: M and N, which differ in two amino acids. The M group possesses Ser1 and Gly5 while the N group has Leu1 and Glu5. Recent data suggest that exposure to toxins can cause mutation or loss of an allele resulting in phenotypic changes. Studies are also beginning to correlate genotype/phenotype with predisposition to cancer and heart disease. Applications Reported: This 6A7M antibody has been reported for use in flow cytometric analysis. Applications Tested: This 6A7M antibody has been pre-titrated and tested bu flow cytometric analysis of human peripheral blood cells. This can be used at 5 µL (0.5 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. eFluor® 450 is an alternative to Pacific Blue®. eFluor® 450 emits at 445 nm and is excited with the Violet laser (405 nm). Please make sure that your instrument is capable of detecting this fluorochome. Excitation: 405 nm; Emission: 445 nm; Laser: Violet Laser. Filtration: 0.2 µm post-manufacturing filtered.
Reactivity
Human
Host
Mouse
Isotype
IgG
Antibody clone number
6A7M
Vial size
100 Tests
Concentration
5 µL/Test
Storage
4° C, store in dark, DO NOT FREEZE!
GYPA protein structure - 48-9884-42 shown in red.