Antibody data
- Antibody Data
- Antigen structure
- References [10]
- Comments [0]
- Validations
- Flow cytometry [1]
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- Product number
- 48-9987-41 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-CD235a (Glycophorin A) Monoclonal Antibody (HIR2 (GA-R2)), eFluor 450, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: The HIR2 monoclonal antibody reacts with human glycophorin A, a sialoglycoprotein expressed by erythroid precursors and mature circulating red cells. Applications Reported: This HIR2 (also GA-R2) antibody has been reported for use in flow cytometric analysis. Applications Tested: This HIR2 (also GA-R2) antibody has been pre-titrated and tested by flow cytometric analysis of diluted normal human peripheral blood cells. Binding of this antibody to red cells at high antibody concentration causes cell agglutination. This can be used at 5 µL (0.03 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. eFluor® 450 is an alternative to Pacific Blue®. eFluor® 450 emits at 445 nm and is excited with the Violet laser (405 nm). Please make sure that your instrument is capable of detecting this fluorochome. Excitation: 405 nm; Emission: 445 nm; Laser: Violet Laser. Filtration: 0.2 µm post-manufacturing filtered.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- HIR2 (GA-R2)
- Vial size
- 25 Tests
- Concentration
- 5 µL/Test
- Storage
- 4° C, store in dark, DO NOT FREEZE!
Submitted references Comprehensive human cell-type methylation atlas reveals origins of circulating cell-free DNA in health and disease.
Platelets confound the measurement of extracellular miRNA in archived plasma.
Genetic Variability Overrides the Impact of Parental Cell Type and Determines iPSC Differentiation Potential.
PPAR-α and glucocorticoid receptor synergize to promote erythroid progenitor self-renewal.
Altered translation of GATA1 in Diamond-Blackfan anemia.
EDAG positively regulates erythroid differentiation and modifies GATA1 acetylation through recruiting p300.
A feedback loop consisting of microRNA 23a/27a and the β-like globin suppressors KLF3 and SP1 regulates globin gene expression.
Terminal differentiation and loss of tumorigenicity of human cancers via pluripotency-based reprogramming.
α-1,6-Fucosyltransferase (FUT8) inhibits hemoglobin production during differentiation of murine and K562 human erythroleukemia cells.
Role of ZBP-89 in human globin gene regulation and erythroid differentiation.
Moss J, Magenheim J, Neiman D, Zemmour H, Loyfer N, Korach A, Samet Y, Maoz M, Druid H, Arner P, Fu KY, Kiss E, Spalding KL, Landesberg G, Zick A, Grinshpun A, Shapiro AMJ, Grompe M, Wittenberg AD, Glaser B, Shemer R, Kaplan T, Dor Y
Nature communications 2018 Nov 29;9(1):5068
Nature communications 2018 Nov 29;9(1):5068
Platelets confound the measurement of extracellular miRNA in archived plasma.
Mitchell AJ, Gray WD, Hayek SS, Ko YA, Thomas S, Rooney K, Awad M, Roback JD, Quyyumi A, Searles CD
Scientific reports 2016 Sep 13;6:32651
Scientific reports 2016 Sep 13;6:32651
Genetic Variability Overrides the Impact of Parental Cell Type and Determines iPSC Differentiation Potential.
Kyttälä A, Moraghebi R, Valensisi C, Kettunen J, Andrus C, Pasumarthy KK, Nakanishi M, Nishimura K, Ohtaka M, Weltner J, Van Handel B, Parkkonen O, Sinisalo J, Jalanko A, Hawkins RD, Woods NB, Otonkoski T, Trokovic R
Stem cell reports 2016 Feb 9;6(2):200-12
Stem cell reports 2016 Feb 9;6(2):200-12
PPAR-α and glucocorticoid receptor synergize to promote erythroid progenitor self-renewal.
Lee HY, Gao X, Barrasa MI, Li H, Elmes RR, Peters LL, Lodish HF
Nature 2015 Jun 25;522(7557):474-7
Nature 2015 Jun 25;522(7557):474-7
Altered translation of GATA1 in Diamond-Blackfan anemia.
Ludwig LS, Gazda HT, Eng JC, Eichhorn SW, Thiru P, Ghazvinian R, George TI, Gotlib JR, Beggs AH, Sieff CA, Lodish HF, Lander ES, Sankaran VG
Nature medicine 2014 Jul;20(7):748-53
Nature medicine 2014 Jul;20(7):748-53
EDAG positively regulates erythroid differentiation and modifies GATA1 acetylation through recruiting p300.
Zheng WW, Dong XM, Yin RH, Xu FF, Ning HM, Zhang MJ, Xu CW, Yang Y, Ding YL, Wang ZD, Zhao WB, Tang LJ, Chen H, Wang XH, Zhan YQ, Yu M, Ge CH, Li CY, Yang XM
Stem cells (Dayton, Ohio) 2014 Aug;32(8):2278-89
Stem cells (Dayton, Ohio) 2014 Aug;32(8):2278-89
A feedback loop consisting of microRNA 23a/27a and the β-like globin suppressors KLF3 and SP1 regulates globin gene expression.
Ma Y, Wang B, Jiang F, Wang D, Liu H, Yan Y, Dong H, Wang F, Gong B, Zhu Y, Dong L, Yin H, Zhang Z, Zhao H, Wu Z, Zhang J, Zhou J, Yu J
Molecular and cellular biology 2013 Oct;33(20):3994-4007
Molecular and cellular biology 2013 Oct;33(20):3994-4007
Terminal differentiation and loss of tumorigenicity of human cancers via pluripotency-based reprogramming.
Zhang X, Cruz FD, Terry M, Remotti F, Matushansky I
Oncogene 2013 May 2;32(18):2249-60, 2260.e1-21
Oncogene 2013 May 2;32(18):2249-60, 2260.e1-21
α-1,6-Fucosyltransferase (FUT8) inhibits hemoglobin production during differentiation of murine and K562 human erythroleukemia cells.
Sasaki H, Toda T, Furukawa T, Mawatari Y, Takaesu R, Shimizu M, Wada R, Kato D, Utsugi T, Ohtsu M, Murakami Y
The Journal of biological chemistry 2013 Jun 7;288(23):16839-47
The Journal of biological chemistry 2013 Jun 7;288(23):16839-47
Role of ZBP-89 in human globin gene regulation and erythroid differentiation.
Woo AJ, Kim J, Xu J, Huang H, Cantor AB
Blood 2011 Sep 29;118(13):3684-93
Blood 2011 Sep 29;118(13):3684-93
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Staining of normal human peripheral blood cells with Mouse IgG2b K Isotype Control eFluor® 450 (Product # 48-4732-82) (blue histogram) or Anti-Human CD235a (Glycophorin A) eFluor® 450 (purple histogram). All non-leukocytes were used for analysis.