60111.1

antibody from STEMCELL Technologies Inc

Targeting: GYPA CD235a, GPA, MN, MNS

Immunocytochemistry (Recommended by provider)

Immunohistochemistry (Recommended by provider)

Flow cytometry (Recommended by provider)

Antibody Data

Product number

60111.1

Provider

STEMCELL Technologies Inc

Product name

Anti-Human CD235ab (Glycophorin A/B) Antibody, Clone HIR2

Antibody type

Monoclonal

Description

The HIR2 (GA-R2) antibody recognizes an epitope common to the N-terminal region of human CD235a (glycophorin A) and CD235b (glycophorin B), homologous type I sialoglycoproteins of the erythrocyte (red blood cell) membrane that bear the antigenic determinants for the MN and Ss blood groups. CD235ab is expressed on early- and late-stage erythroblasts, mature erythrocytes, and erythroid cell lines such as K562 and HEL, but not on other cell types. CD235a is abundantly expressed whereas CD235b is a relatively minor membrane component. The proteins are believed to provide a large mucin-like surface to erythrocytes that acts to minimize aggregation in the circulation. The HIR2 antibody binds with higher affinity to CD235a than CD235b, and agglutinates untreated erythrocytes.

Reactivity

Human

Host

Human

Isotype

IgG

Vial size

25 µg

Storage

2 - 8°C