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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Western blot [1]
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- Product number
- 600-401-916 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SPANX Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 1 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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- Invitrogen Antibodies (provider)
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- Experimental details
- Western blot using Rocklands affinity purified anti-SPANX (pan) antibody shows detection of a band at ~17 kDa corresponding to SPANX-C present in a nuclear extract from VWM105 cells (left panel, arrowhead). VWM105 cells are derived from a human melanoma and are positive for SPANX proteins. Lane 2 shows reactivity with a purified recombinant SPANX-C fusion protein. The right panel shows similar reactivity with purified recombinant SPANX-B, SPANX-C and SPANX-N proteins. Proteins were separated by SDS-PAGE, transferred to nitrocellulose, and probed with the primary antibody diluted to 1:1,000. IRDye800 conjugated Gt-a-Rabbit IgG [H&L] MX (611-132-122) was used (left). IRDye is a trademark of LI-COR, Inc. Size estimation was made by comparison to prestained MW markers as indicated. Personal Communication. Vladimir Larionov, NIH, CCR, Bethesda, MD.
