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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [9]
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- Validations
- Western blot [2]
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- Product number
- AF5366 - Provider product page
- Provider
- R&D Systems
- Product name
- Human/Mouse/Rat MuRF1/TRIM63 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects human, mouse, and rat MuRF1/TRIM63 in direct ELISAs and Western blots.
- Reactivity
- Human, Mouse, Rat
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
Q969Q1- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Buyang Huanwu Tang improves denervation-dependent muscle atrophy by increasing ANGPTL4, and increases NF-κB and MURF1 levels.
The Calcineurin-FoxO-MuRF1 signaling pathway regulates myofibril integrity in cardiomyocytes.
Effect of 20-Hydroxyecdysone on Proteolytic Regulation in Skeletal Muscle Atrophy.
Effect of 20-Hydroxyecdysone on Proteolytic Regulation in Skeletal Muscle Atrophy.
Masseter muscle myofibrillar protein synthesis and degradation in an experimental critical illness myopathy model.
The posterior cricoarytenoid muscle is spared from MuRF1-mediated muscle atrophy in mice with acute lung injury.
TRAF6 inhibition rescues dexamethasone-induced muscle atrophy.
Protein synthesis in skeletal muscle of neonatal pigs is enhanced by administration of β-hydroxy-β-methylbutyrate.
Muscle RING finger-1 attenuates IGF-I-dependent cardiomyocyte hypertrophy by inhibiting JNK signaling.
Zhou L, Huang Y, Xie H, Mei X
Molecular medicine reports 2018 Mar;17(3):3674-3680
Molecular medicine reports 2018 Mar;17(3):3674-3680
The Calcineurin-FoxO-MuRF1 signaling pathway regulates myofibril integrity in cardiomyocytes.
Shimizu H, Langenbacher AD, Huang J, Wang K, Otto G, Geisler R, Wang Y, Chen JN
eLife 2017 Aug 19;6
eLife 2017 Aug 19;6
Effect of 20-Hydroxyecdysone on Proteolytic Regulation in Skeletal Muscle Atrophy.
Hirunsai M, Yimlamai T, Suksamrarn A
In vivo (Athens, Greece) 2016 11-12;30(6):869-877
In vivo (Athens, Greece) 2016 11-12;30(6):869-877
Effect of 20-Hydroxyecdysone on Proteolytic Regulation in Skeletal Muscle Atrophy.
Hirunsai M, Yimlamai T, Suksamrarn A
In vivo (Athens, Greece) 2016 11-12;30(6):869-877
In vivo (Athens, Greece) 2016 11-12;30(6):869-877
Masseter muscle myofibrillar protein synthesis and degradation in an experimental critical illness myopathy model.
Akkad H, Corpeno R, Larsson L
PloS one 2014;9(4):e92622
PloS one 2014;9(4):e92622
The posterior cricoarytenoid muscle is spared from MuRF1-mediated muscle atrophy in mice with acute lung injury.
Files DC, Xiao K, Zhang T, Liu C, Qian J, Zhao W, Morris PE, Delbono O, Feng X
PloS one 2014;9(1):e87587
PloS one 2014;9(1):e87587
TRAF6 inhibition rescues dexamethasone-induced muscle atrophy.
Sun H, Gong Y, Qiu J, Chen Y, Ding F, Zhao Q
International journal of molecular sciences 2014 Jun 20;15(6):11126-41
International journal of molecular sciences 2014 Jun 20;15(6):11126-41
Protein synthesis in skeletal muscle of neonatal pigs is enhanced by administration of β-hydroxy-β-methylbutyrate.
Wheatley SM, El-Kadi SW, Suryawan A, Boutry C, Orellana RA, Nguyen HV, Davis SR, Davis TA
American journal of physiology. Endocrinology and metabolism 2014 Jan 1;306(1):E91-9
American journal of physiology. Endocrinology and metabolism 2014 Jan 1;306(1):E91-9
Muscle RING finger-1 attenuates IGF-I-dependent cardiomyocyte hypertrophy by inhibiting JNK signaling.
Wadosky KM, Rodríguez JE, Hite RL, Min JN, Walton BL, Willis MS
American journal of physiology. Endocrinology and metabolism 2014 Apr 1;306(7):E723-39
American journal of physiology. Endocrinology and metabolism 2014 Apr 1;306(7):E723-39
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human/Mouse/Rat MuRF1 by Western Blot. Western blot shows lysates of human heart, mouse heart, and rat muscle tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat MuRF1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5366) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for MuRF1 at approximately 41-44 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human MuRF1/TRIM63 by Simple WesternTM. Simple Western lane view shows lysates of human heart tissue, loaded at 0.2 mg/mL. A specific band was detected for MuRF1/TRIM63 at approximately 46 kDa (as indicated) using 10 µg/mL of Goat Anti-Human/Mouse/Rat MuRF1/TRIM63 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5366) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
