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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Other assay [1]
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- Product number
- PA5-57274 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- MRPS18A Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: PSELQRELQR LSPLPRHSGL LPNHRPRLPE GVVPKSKPQL NRYLTRWAPG SVKPIYKKGP RWNRVRMPVG SPLLRDNVCY SRTPWKLYH
- Concentration
- 0.1 mg/mL
Submitted references Inhibition of mitochondrial translation suppresses glioblastoma stem cell growth.
Sighel D, Notarangelo M, Aibara S, Re A, Ricci G, Guida M, Soldano A, Adami V, Ambrosini C, Broso F, Rosatti EF, Longhi S, Buccarelli M, D'Alessandris QG, Giannetti S, Pacioni S, Ricci-Vitiani L, Rorbach J, Pallini R, Roulland S, Amunts A, Mancini I, Modelska A, Quattrone A
Cell reports 2021 Apr 27;35(4):109024
Cell reports 2021 Apr 27;35(4):109024
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of MRPS18A in Lane 1: Marker (kDa) 230, 130, 95, 72, 56, 36, 28, 17, 11; Lane 2: Human cell line RT-4; Lane 3: Human cell line U-251MG sp. Samples were probed using a MRPS18A Polyclonal Antibody (Product # PA5-57274).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of MRPS18A in human liver tissue shows strong cytoplasmic positivity in hepatocytes. Samples were probed using a MRPS18A Polyclonal Antibody (Product # PA5-57274).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 6 Genetic inhibition of mitochondrial translation suppresses GSC growth, recapitulating Q/D effects (A) Competition assay in VIPI cells transduced with viruses expressing sgRNAs for target genes. Data were normalized to day 1. n = 4 biological replicates, mean +- SD. (B) Percentage of insertions or deletions (indels) analyzed by decomposition (tracking of indels by decomposition [TIDE]) analysis in Cas9-expressing COMI and VIPI cells following lentiviral transduction of the sgRNAs selected in (A) (sgTUFM_1 and sgMRPS18A_1). n = 3-4 biological replicates, mean +- SD. (C) Effects of TUFM and MRPS18A knockout on TUFM, MRPS18A, and beta-tubulin proteins in COMI and VIPI cells, as assayed by immunoblotting. One representative result is shown; n = 2 biological replicates. (D) Effects of TUFM and MRPS18A knockout on COX1, COX4, and beta-tubulin proteins in COMI and VIPI cells, as assayed by immunoblotting. One representative result is shown; n = 2 biological replicates. (E) Effects of TUFM and MRPS18A knockout on COMI and VIPI cells grown in suspension. Shown are example images from days 0, 4, 10, and 15; scale bar, 200 mum. (F) Sphere area measured over the course of the 15-day experiment. The data were normalized to day 0. n = 15 technical replicates, mean +- SEM. One representative experiment is shown; n = 3 biological replicates. (G) Effects of TUFM and MRPS18A knockout on COMI and VIPI proliferation when grown as adherent cultures. The data were normalized to day 0. n =
