PA5-57274

antibody from Invitrogen Antibodies

Targeting: MRPS18A FLJ10548, MRPS18-3

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-57274

Provider

Invitrogen Antibodies

Product name

MRPS18A Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Immunogen sequence: PSELQRELQR LSPLPRHSGL LPNHRPRLPE GVVPKSKPQL NRYLTRWAPG SVKPIYKKGP RWNRVRMPVG SPLLRDNVCY SRTPWKLYH Highest antigen sequence identity to the following orthologs: Mouse - 65%, Rat - 64%.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

0.1 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

Inhibition of mitochondrial translation suppresses glioblastoma stem cell growth.

Sighel D, Notarangelo M, Aibara S, Re A, Ricci G, Guida M, Soldano A, Adami V, Ambrosini C, Broso F, Rosatti EF, Longhi S, Buccarelli M, D'Alessandris QG, Giannetti S, Pacioni S, Ricci-Vitiani L, Rorbach J, Pallini R, Roulland S, Amunts A, Mancini I, Modelska A, Quattrone A
Cell reports 2021 Apr 27;35(4):109024

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of MRPS18A in Lane 1: Marker (kDa) 230, 130, 95, 72, 56, 36, 28, 17, 11; Lane 2: Human cell line RT-4; Lane 3: Human cell line U-251MG sp. Samples were probed using a MRPS18A Polyclonal Antibody (Product # PA5-57274).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of MRPS18A in human liver tissue shows strong cytoplasmic positivity in hepatocytes. Samples were probed using a MRPS18A Polyclonal Antibody (Product # PA5-57274).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 6 Genetic inhibition of mitochondrial translation suppresses GSC growth, recapitulating Q/D effects (A) Competition assay in VIPI cells transduced with viruses expressing sgRNAs for target genes. Data were normalized to day 1. n = 4 biological replicates, mean +- SD. (B) Percentage of insertions or deletions (indels) analyzed by decomposition (tracking of indels by decomposition [TIDE]) analysis in Cas9-expressing COMI and VIPI cells following lentiviral transduction of the sgRNAs selected in (A) (sgTUFM_1 and sgMRPS18A_1). n = 3-4 biological replicates, mean +- SD. (C) Effects of TUFM and MRPS18A knockout on TUFM, MRPS18A, and beta-tubulin proteins in COMI and VIPI cells, as assayed by immunoblotting. One representative result is shown; n = 2 biological replicates. (D) Effects of TUFM and MRPS18A knockout on COX1, COX4, and beta-tubulin proteins in COMI and VIPI cells, as assayed by immunoblotting. One representative result is shown; n = 2 biological replicates. (E) Effects of TUFM and MRPS18A knockout on COMI and VIPI cells grown in suspension. Shown are example images from days 0, 4, 10, and 15; scale bar, 200 mum. (F) Sphere area measured over the course of the 15-day experiment. The data were normalized to day 0. n = 15 technical replicates, mean +- SEM. One representative experiment is shown; n = 3 biological replicates. (G) Effects of TUFM and MRPS18A knockout on COMI and VIPI proliferation when grown as adherent cultures. The data were normalized to day 0. n =