15463-1-AP

antibody from Proteintech Group

Targeting: MAPKAP1 MGC2745, MIP1, SIN1

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

15463-1-AP

Provider

Proteintech Group

Proper citation

Proteintech Cat#15463-1-AP, RRID:AB_10598466

Product name

MAPKAP1 antibody

Antibody type

Polyclonal

Description

MAPKAP1 antibody (Cat. #15463-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IF, IHC, IP, WB,ELISA.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Conjugate

Unconjugated

Isotype

IgG

Vial size

20ul, 150ul

References

Submitted references

SIN1 facilitates glioma progression and is associated with the KRAS/ERK pathway.

Cao H, Yan Z, Li M, Wang J, Zhang H, Cheng Y, Sun J, Ren J, Yang D
Journal of neuro-oncology 2025 Nov;175(2):697-711

---

Ibudilast Protects Retinal Bipolar Cells From Excitotoxic Retinal Damage and Activates the mTOR Pathway.

Hamadmad S, Heisler-Taylor T, Goswami S, Hawthorn E, Chaurasia S, Martini D, Summitt D, Zatari A, Shalash R, Sohail M, Urbanski EG, Bernstein K, Racine J, Satoskar A, El-Hodiri HM, Fischer AJ, Cebulla CM
Glia 2025 May;73(5):905-927

---

Phosphorylation of USP33 by CDK1 stabilizes the mTORC2 component SIN1.

Wen Y, Zhang C, Liang M, Yang X, Zeng H, Wan R, Ma X, Huang L, Li M, Zhang Q, Li L, Qin S, Liu T
Cell death & disease 2025 Jul 22;16(1):543

---

Development of a SIN1 targeting inhibitor as a novel therapeutic approach for the treatment of malignancies.

Beauchamp EM, Jain A, Clutter M, Nahotko D, Lopez Fajardo E, Vagadia PP, Dunne SF, Mina A, Fischietti M, Oku CV, Blyth GT, Mishra RK, Karras E, Radecki SG, Lantz C, Addepalli S, Modi M, Zou L, Cho BK, Goo YA, Rossoff J, Fish EN, Saleiro D, Schiltz GE, Platanias LC
Molecular cancer therapeutics 2025 Dec 22;

---

CRLF1 bridges AKT and mTORC2 through SIN1 to inhibit pyroptosis and enhance chemo-resistance in ovarian cancer.

Xiang C, Chen L, Zhu S, Chen Y, Huang H, Yang C, Chi Y, Wang Y, Lei Y, Cai X
Cell death & disease 2024 Sep 10;15(9):662

---

Nup358 restricts ER-mitochondria connectivity by modulating mTORC2/Akt/GSK3β signalling.

Kalarikkal M, Saikia R, Oliveira L, Bhorkar Y, Lonare A, Varshney P, Dhamale P, Majumdar A, Joseph J
EMBO reports 2024 Oct;25(10):4226-4251

---

Overexpression of microRNA-611 inhibits TGF-β-induced epithelial-mesenchymal transition and migration in lung cancer cells through MAPKAP1.

Ke B, Huang Y, Gong Y, Zhong H, Shi L
Cellular signalling 2024 Nov;123:111357

---

Ibudilast Protects Retinal Bipolar Cells from Excitotoxic Retinal Damage and Activates the mTOR Pathway.

Hamadmad S, Heisler-Taylor T, Goswami S, Hawthorn E, Chaurasia S, Martini D, Summitt D, Zaatari A, Urbanski EG, Bernstein K, Racine J, Satoskar A, El-Hodiri HM, Fischer AJ, Cebulla CM
bioRxiv : the preprint server for biology 2024 Mar 20;

---

Host-derived oxidized phospholipids initiate effector-triggered immunity fostering lethality upon microbial encounter.

Gioia MD, Poli V, Tan PJ, Spreafico R, Chu A, Cuenca AG, Gordts PL, Pandolfi L, Meloni F, Witztum JL, Chou J, Springstead JR, Zanoni I
bioRxiv : the preprint server for biology 2023 Nov 21;

---

Mitochondrial dynamics define muscle fiber type by modulating cellular metabolic pathways.

Yasuda T, Ishihara T, Ichimura A, Ishihara N
Cell reports 2023 May 30;42(5):112434

---

Quantitative sequencing using BID-seq uncovers abundant pseudouridines in mammalian mRNA at base resolution.

Dai Q, Zhang LS, Sun HL, Pajdzik K, Yang L, Ye C, Ju CW, Liu S, Wang Y, Zheng Z, Zhang L, Harada BT, Dou X, Irkliyenko I, Feng X, Zhang W, Pan T, He C
Nature biotechnology 2023 Mar;41(3):344-354

---

Hepatic DNAJB9 Drives Anabolic Biasing to Reduce Steatosis and Obesity.

Sun F, Liao Y, Qu X, Xiao X, Hou S, Chen Z, Huang H, Li P, Fu S
Cell reports 2020 Feb 11;30(6):1835-1847.e9

---

The Functional Proximal Proteome of Oncogenic Ras Includes mTORC2.

Kovalski JR, Bhaduri A, Zehnder AM, Neela PH, Che Y, Wozniak GG, Khavari PA
Molecular cell 2019 Feb 21;73(4):830-844.e12

Western blot

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

mouse kidney tissue were subjected to SDS PAGE followed by western blot with 15463-1-AP(MAPKAP1 antibody) at dilution of 1:500

Sample type

tissue

Immunocytochemistry

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunofluorescent analysis of HepG2 cells, using MAPKAP1 antibody 15463-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

Sample type

cell line

Immunohistochemistry

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunohistochemical of paraffin-embedded human kidney using 15463-1-AP(MAPKAP1 antibody) at dilution of 1:100 (under 10x lens)

Sample type

tissue

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunohistochemical of paraffin-embedded human kidney using 15463-1-AP(MAPKAP1 antibody) at dilution of 1:100 (under 40x lens)

Sample type

tissue