15463-1-AP

antibody from Invitrogen Antibodies

Targeting: MAPKAP1 MGC2745, MIP1, SIN1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

15463-1-AP

Provider

Invitrogen Antibodies

Product name

MAPKAP1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: MAFLDNPTI ILAHIRQSHV TSDDTGMCEM VLIDHDVDLE KIHPPSMPGD SGSEIQGSNG ETQGYVYAQS VDITSSWDFG IRRRSNTAQR LERLRKERQN QIKCKNIQWK ERNSKQSAQE LKSLFEKKSL KEKPPISGKQ SILSVRLEQC PLQLNNPFNE YSKFDGKGHV GTTATKKIDV YLPLHSSQDR LLPMTVVTMA SARVQDLIGL ICWQYTSEGR EPKLNDNVSA YCLHIAEDDG EVDTDFPPLD SNEPIHKFGF STLALVEKYS SPGLTSKESL FVRINAAHGF SLIQVDNTKV TMKEILLKAV KRRKGSQKVS GSRAD (1-324 aa encoded by BC002326)

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.34 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Mouse kidney tissue were subjected to SDS PAGE followed by western blot with 15463-1-AP (MAPKAP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HeLa cells were subjected to SDS PAGE followed by western blot with 15463-1-AP (MAPKAP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using MAPKAP1 antibody (Product # 15463-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of HepG2 cells, using MAPKAP1 antibody 15463-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IGG (red).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human kidney using 15463-1-AP (MAPKAP1 antibody) at dilution of 1:100 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human kidney using 15463-1-AP (MAPKAP1 antibody) at dilution of 1:100 (under 40x lens).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-MAPKAP1 (IP:15463-1-AP, 4ug; Detection:15463-1-AP 1:500) with mouse kidney tissue lysate 4000ug.