Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunohistochemistry [1]
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- Product number
- PA5-20362 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- MAPKAP1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- A suggested positive control is human skeletal muscle tissue lysate. PA5-20362 can be used with blocking peptide PEP-0587.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 1 mg/mL
- Storage
- Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of human skeletal muscle tissue lysate using a MAPKAP1 polyclonal antibody (Product # PA5-20362) at (A) 1 and (B) 2 µg/mL.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of MAPKAP1 in human skeletal muscle tissue lysate with MAPKAP1 Polyclonal Antibody (Product # PA5-20362) at (A) 1 and (B) 2 µg/mL.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-MAPKAP1 Polyclonal Antibody (Product # PA5-20362) and a 65 and 55 kDa band corresponding to Target of rapamycin complex 2 subunit MAPKAP1 was observed across the cell lines and the tissue lysates except the pancreas which is a negative model. Whole cell extracts (30 µg lysate) of HeLa (Lane 1), HEK-293 (Lane 2), Jurkat (Lane 3) in Fig a and Mouse Lung (Lane 1), Rat Lung (Lane 2), Mouse Skeletal Muscle (Lane 3), Mouse Kidney (Lane 4), Rat Kidney (Lane 5), Mouse Adipose (Lane 6), Mouse Liver (Lane 7), Mouse Pancreas (Lane 8) in Fig b were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23002) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036,1:10000) using the iBright™ FL1500 Imaging System (Product # A44115). Chemiluminescent detection was performed using SuperSignal™ West Dura Extended Duration Substrate (Product # 34076).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of MAPKAP1 in human skeletal muscle tissue with MAPKAP1 Polyclonal Antibody (Product # PA5-20362) at 5 µg/mL.