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ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Immunohistochemistry [1]
- Flow cytometry [1]
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Validation data
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- Product number
- MAB5854 - Provider product page
- Provider
- R&D Systems
- Product name
- Human Galanin Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human Galanin in direct ELISAs.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
P22466- Isotype
- IgG
- Antibody clone number
- 581403
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Galanin in Human Placenta. Galanin was detected in immersion fixed paraffin-embedded sections of human placenta using Human Galanin Monoclonal Antibody (Catalog # MAB5854) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to syncytiotrophoblast cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Galanin in U-87MG Human Cell Line by Flow Cytometry. U-87 MG human glioblastoma/astrocytoma cell line was stained with Human Galanin Monoclonal Antibody (Catalog # MAB5854, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by PerCP-conjugated Anti-Mouse IgG F(ab')2 Secondary Antibody (Catalog # F0114). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.
