PA5-55656

antibody from Invitrogen Antibodies

Targeting: SORBS1 CAP, FLJ12406, KIAA1296, ponsin, SH3D5, sh3p12

Western blot

Immunohistochemistry

Antibody data

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Validation data

Product number: PA5-55656 - Provider product page
Provider: Invitrogen Antibodies
Product name: SORBS1 Polyclonal Antibody
Antibody type: Polyclonal
Antigen: Recombinant full-length protein
Description: Immunogen sequence: DSSPLLNEVS SSLIGTDSQA FPSVSKPSSA YPSTTIVNPT IVLLQHNREQ QKRLSSLSDP VSERRVGEQD SAPTQEKPTS PGKAIEKRAK DDSRRVVKST QDLSDVSMDE VGIPLRNTER SKDWYKTMFK QIHKLNR Highest antigen sequence identity to the following orthologs: Mouse - 84%, Rat - 85%.
Reactivity: Human
Host: Rabbit
Isotype: IgG
Vial size: 100 µL
Concentration: 0.3 mg/mL
Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

SORBS1 protein structure - PA5-55656 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 3. MiR-3646 targeted SORBS1 by binding to its 3'UTR oligonucleotide sequence. (a) TargetScan Human 7.2 was used to predict the potential binding site between miR-3646 and its targeted gene. (b) Potential binding between miR-3646 and the 3'UTR of SORBS1 mRNA was demonstrated by the luciferase reporter assay. *P < 0.05, **P < 0.001, compared with corresponding miR-NC group. (c) The interaction between SORBS1 and miR-3646 was evaluated by RNA pull-down analysis. **P < 0.001, compared with Bio-NC group. (d) Negative correlation between miR-3646 and SORBS1 expression was identified by Pearson's correlation analysis. (e) SORBS1 mRNA expression in LUAD cell lines was analyzed by qRT-PCR. Except for Beas-2B cell line, the others are LUAD cell lines. LUAD cell lines including A549, PC9, Calu-3, SPC-A1 and H1975 showed lower expression of SORBS1 mRNA compared to Beas-2B cell line. **P < 0.001, compared with Beas-2B cell line. (f) SORBS1 protein expression in LUAD cell lines was analyzed by Western blotting. Except for Beas-2B cell line, the others are LUAD cell lines. LUAD cell lines including A549, PC9, Calu-3, SPC-A1 and H1975 showed lower expression of SORBS1 protein compared to Beas-2B cell line. The error bars were derived from 3 independent parallel experiments. **P < 0.001, compared with Beas-2B cell line.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 4. MiR-3646 advanced LUAD by targeting SORBS1 mRNA. (a) Western blot analysis of SORBS1 was used to evaluate the transfection efficiency of A549 and PC9 cells undergoing transfection with miR-3646 mimic (mimic group), OE-SORBS1 (OE), miR-3646 mimic plus OE-SORBS1 (mimic+OE) or negative control (NC), with GADPH as the internal control. Blank group indicated untreated cells. (b) The cell viability of the transfected A549 and PC9 cells was measured by CCK-8 assay. (c) The proliferation of the transfected A549 and PC9 cells was measured by BrdU incorporation assay. (d) Cell-matrix adhesion assay was used to determine the adhesion ability of transfected A549 and PC9 cells. (e) Caspase-3 activity assay was conducted to measure A549 and PC9 cell apoptosis. (f) Western blots analysis of Bcl-2 and Bax protein levels in A549 and PC9 cells. (The error bars were derived from 3 independent parallel experiments). (g) Western blots analysis of p-JNK and JNK. The error bars were derived from 3 independent parallel experiments. *P < 0.05, **P < 0.001, compared with blank group; ##P < 0.001, compared with OE-SORBS1 group.