11724-1-AP

antibody from Proteintech Group

Targeting: LIN28A CSDD1, FLJ12457, LIN-28, LIN28, ZCCHC1

Provider product page for 11724-1-AP

Western blot

ELISA

Immunocytochemistry

Immunoprecipitation

Immunohistochemistry

Antibody data

Antibody Data
Antigen structure
References [50]
Comments [0]
Validations
Western blot [1]
Immunocytochemistry [2]
Immunoprecipitation [1]
Immunohistochemistry [1]

Submit

Product number: 11724-1-AP - Provider product page
Provider: Proteintech Group
Proper citation: Proteintech Cat#11724-1-AP, RRID:AB_2135039
Product name: LIN28 antibody
Antibody type: Polyclonal
Description: KD/KO validated LIN28 antibody (Cat. #11724-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IF, IHC, IP, WB, ELISA.
Reactivity: Human, Mouse, Rat
Host: Rabbit
Conjugate: Unconjugated
Isotype: IgG
Vial size: 20ul, 150ul

LIN28A protein structure - 11724-1-AP shown in red.

Submitted references Inhibition of Let-7b-5p maturation by LIN28A promotes thermal skin damage repair after burn injury.

Zhou S, Guo L, Cui X, Zhang X, Yang Y, Zhang M, Zhang P
Cellular signalling 2024 Aug;120:111217

miR-188-5p silencing improves cerebral ischemia/reperfusion injury by targeting Lin28a.

Hou D, Pei C, Yu D, Yang G
Metabolic brain disease 2023 Oct;38(7):2327-2338

Hypoxic preconditioned MSCs-derived small extracellular vesicles for photoreceptor protection in retinal degeneration.

Sun Y, Sun Y, Chen S, Yu Y, Ma Y, Sun F
Journal of nanobiotechnology 2023 Nov 25;21(1):449

Cocktails of NSAIDs and 17α Ethinylestradiol at Environmentally Relevant Doses in Drinking Water Alter Puberty Onset in Mice Intergenerationally.

Philibert P, Déjardin S, Girard M, Durix Q, Gonzalez AA, Mialhe X, Tardat M, Poulat F, Boizet-Bonhoure B
International journal of molecular sciences 2023 Mar 20;24(6)

Alternative transcribed 3' isoform of long non-coding RNA Malat1 inhibits mouse retinal oxidative stress.

Ghanam AR, Ke S, Wang S, Elgendy R, Xie C, Wang S, Zhang R, Wei M, Liu W, Cao J, Zhang Y, Zhang Z, Xue T, Zheng Y, Song X
iScience 2023 Jan 20;26(1):105740

Intergenerational effects on fertility in male and female mice after chronic exposure to environmental doses of NSAIDs and 17α-ethinylestradiol mixtures.

Philibert P, Stévant I, Déjardin S, Girard M, Sellem E, Durix Q, Messager A, Gonzalez AA, Mialhe X, Pruvost A, Poulat F, Boizet-Bonhoure B
Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association 2023 Dec;182:114085

Hmmr Acts as a Key Regulator in the ADSCs Proliferation and Mitosis.

Zhang X, Huang D, Li K, Han C, Li H, Li C, Liu L
Stem cell reviews and reports 2023 Aug;19(6):1937-1953

Exosomal hsa_circ_000200 as a potential biomarker and metastasis enhancer of gastric cancer via miR-4659a/b-3p/HBEGF axis.

Huang XJ, Wang Y, Wang HT, Liang ZF, Ji C, Li XX, Zhang LL, Ji RB, Xu WR, Jin JH, Qian H
Cancer cell international 2023 Aug 1;23(1):151

Extracellular vesicles from neurons promote neural induction of stem cells through cyclin D1.

Song L, Tian X, Schekman R
The Journal of cell biology 2021 Sep 6;220(9)

ETV4 promotes breast cancer cell stemness by activating glycolysis and CXCR4-mediated sonic Hedgehog signaling.

Zhu T, Zheng J, Zhuo W, Pan P, Li M, Zhang W, Zhou H, Gao Y, Li X, Liu Z
Cell death discovery 2021 May 29;7(1):126

Defective metabolic programming impairs early neuronal morphogenesis in neural cultures and an organoid model of Leigh syndrome.

Inak G, Rybak-Wolf A, Lisowski P, Pentimalli TM, Jüttner R, Glažar P, Uppal K, Bottani E, Brunetti D, Secker C, Zink A, Meierhofer D, Henke MT, Dey M, Ciptasari U, Mlody B, Hahn T, Berruezo-Llacuna M, Karaiskos N, Di Virgilio M, Mayr JA, Wortmann SB, Priller J, Gotthardt M, Jones DP, Mayatepek E, Stenzel W, Diecke S, Kühn R, Wanker EE, Rajewsky N, Schuelke M, Prigione A
Nature communications 2021 Mar 26;12(1):1929

Non-microRNA binding competitively inhibits LIN28 regulation.

Tan FE, Sathe S, Wheeler EC, Yeo GW
Cell reports 2021 Aug 10;36(6):109517

Generation of human induced pluripotent stem cell line from Alzheimer's disease patient with PSEN2 N141I mutation using integration-free non-viral method.

Murti H, Pieknell K, Bachtiar I, Sulistio YA, Lee SH
Stem cell research 2020 Jun 29;47:101892

Intergenerational effects on mouse sperm quality after in utero exposure to acetaminophen and ibuprofen.

Rossitto M, Marchive C, Pruvost A, Sellem E, Ghettas A, Badiou S, Sutra T, Poulat F, Philibert P, Boizet-Bonhoure B
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2019 Jan;33(1):339-357

Expression of RNA-binding protein LIN28 in classic gastric hepatoid carcinomas, gastric fetal type gastrointestinal adenocarcinomas, and hepatocellular carcinomas: An immunohistochemical study with comparison to SALL4, alpha-fetoprotein, glypican-3, and Hep Par1.

Zhao M, Sun L, Lai JZ, Shi H, Mei K, He X, Jin X, Lai J, Cao D
Pathology, research and practice 2018 Oct;214(10):1707-1712

The lncRNA MACC1-AS1 promotes gastric cancer cell metabolic plasticity via AMPK/Lin28 mediated mRNA stability of MACC1.

Zhao Y, Liu Y, Lin L, Huang Q, He W, Zhang S, Dong S, Wen Z, Rao J, Liao W, Shi M
Molecular cancer 2018 Mar 6;17(1):69

LIN28A inhibits lysosome‑associated membrane glycoprotein 1 protein expression in embryonic stem and bladder cancer cells.

Pan P, Chen T, Zhang Y, Qi Z, Qin J, Cui G, Guo X
Molecular medicine reports 2018 Jul;18(1):399-406

Rational Development of A Polycistronic Plasmid with A CpG-Free Bacterial Backbone as A Potential Tool for Direct Reprogramming.

Dormiani K, Mir Mohammad Sadeghi H, Sadeghi-Aliabadi H, Forouzanfar M, Baharvand H, Ghaedi K, Nasr-Esfahani MH
Cell journal 2017 Winter;18(4):565-581

Ythdc2 is an N(6)-methyladenosine binding protein that regulates mammalian spermatogenesis.

Hsu PJ, Zhu Y, Ma H, Guo Y, Shi X, Liu Y, Qi M, Lu Z, Shi H, Wang J, Cheng Y, Luo G, Dai Q, Liu M, Guo X, Sha J, Shen B, He C
Cell research 2017 Sep;27(9):1115-1127

Analysis of putative biomarkers of undifferentiated spermatogonia in dog testis.

Lee WY, Park HJ, Lee R, Lee JH, Jhun H, Hur TY, Song H
Animal reproduction science 2017 Oct;185:174-180

Mcl-1 stabilization confers resistance to taxol in human gastric cancer.

Shuang W, Hou L, Zhu Y, Li Q, Hu W
Oncotarget 2017 Oct 10;8(47):82981-82990

Human iPSC-Derived Neural Progenitors Are an Effective Drug Discovery Model for Neurological mtDNA Disorders.

Lorenz C, Lesimple P, Bukowiecki R, Zink A, Inak G, Mlody B, Singh M, Semtner M, Mah N, Auré K, Leong M, Zabiegalov O, Lyras EM, Pfiffer V, Fauler B, Eichhorst J, Wiesner B, Huebner N, Priller J, Mielke T, Meierhofer D, Izsvák Z, Meier JC, Bouillaud F, Adjaye J, Schuelke M, Wanker EE, Lombès A, Prigione A
Cell stem cell 2017 May 4;20(5):659-674.e9

Linc-DYNC2H1-4 promotes EMT and CSC phenotypes by acting as a sponge of miR-145 in pancreatic cancer cells.

Gao Y, Zhang Z, Li K, Gong L, Yang Q, Huang X, Hong C, Ding M, Yang H
Cell death & disease 2017 Jul 13;8(7):e2924

Rapid establishment of the European Bank for induced Pluripotent Stem Cells (EBiSC) - the Hot Start experience.

De Sousa PA, Steeg R, Wachter E, Bruce K, King J, Hoeve M, Khadun S, McConnachie G, Holder J, Kurtz A, Seltmann S, Dewender J, Reimann S, Stacey G, O'Shea O, Chapman C, Healy L, Zimmermann H, Bolton B, Rawat T, Atkin I, Veiga A, Kuebler B, Serano BM, Saric T, Hescheler J, Brüstle O, Peitz M, Thiele C, Geijsen N, Holst B, Clausen C, Lako M, Armstrong L, Gupta SK, Kvist AJ, Hicks R, Jonebring A, Brolén G, Ebneth A, Cabrera-Socorro A, Foerch P, Geraerts M, Stummann TC, Harmon S, George C, Streeter I, Clarke L, Parkinson H, Harrison PW, Faulconbridge A, Cherubin L, Burdett T, Trigueros C, Patel MJ, Lucas C, Hardy B, Predan R, Dokler J, Brajnik M, Keminer O, Pless O, Gribbon P, Claussen C, Ringwald A, Kreisel B, Courtney A, Allsopp TE
Stem cell research 2017 Apr;20:105-114

Activation of the Lin28/let-7 Axis by Loss of ESE3/EHF Promotes a Tumorigenic and Stem-like Phenotype in Prostate Cancer.

Albino D, Civenni G, Dallavalle C, Roos M, Jahns H, Curti L, Rossi S, Pinton S, D'Ambrosio G, Sessa F, Hall J, Catapano CV, Carbone GM
Cancer research 2016 Jun 15;76(12):3629-43

JMJD1A promotes tumorigenesis and forms a feedback loop with EZH2/let-7c in NSCLC cells.

Zhan M, Wen F, Liu L, Chen Z, Wei H, Zhou H
Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine 2016 Aug;37(8):11237-47

RNA-binding protein LIN28 is a sensitive marker of pediatric yolk sac tumors.

Feng S, Huang S, Tong Y, Chen Z, Shen D, Wu D, Lai XH, Chen X
Pediatric surgery international 2016 Aug;32(8):819-25

LIN28A Modulates Splicing and Gene Expression Programs in Breast Cancer Cells.

Yang J, Bennett BD, Luo S, Inoue K, Grimm SA, Schroth GP, Bushel PR, Kinyamu HK, Archer TK
Molecular and cellular biology 2015 Sep;35(18):3225-43

Generation of iPSC lines from primary human chorionic villi cells.

Lichtner B, Matz P, Adjaye J
Stem cell research 2015 Nov;15(3):697-9

Expression of LIN28 and its regulatory microRNAs in adult adrenocortical cancer.

Faria AM, Sbiera S, Ribeiro TC, Soares IC, Mariani BM, Freire DS, de Sousa GR, Lerario AM, Ronchi CL, Deutschbein T, Wakamatsu A, Alves VA, Zerbini MC, Mendonca BB, Fragoso MC, Latronico AC, Fassnacht M, Almeida MQ
Clinical endocrinology 2015 Apr;82(4):481-8

Lin28 promotes Her2 expression and Lin28/Her2 predicts poorer survival in gastric cancer.

Wang Q, Zhou J, Guo J, Teng R, Shen J, Huang Y, Xie S, Wei Q, Zhao W, Chen W, Yuan X, Chen Y, Wang L
Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine 2014 Nov;35(11):11513-21

Divergent LIN28-mRNA associations result in translational suppression upon the initiation of differentiation.

Tan SM, Altschuler G, Zhao TY, Ang HS, Yang H, Lim B, Vardy L, Hide W, Thomson AM, Lareu RR
Nucleic acids research 2014 Jul;42(12):7997-8007

LSD1 regulates pluripotency of embryonic stem/carcinoma cells through histone deacetylase 1-mediated deacetylation of histone H4 at lysine 16.

Yin F, Lan R, Zhang X, Zhu L, Chen F, Xu Z, Liu Y, Ye T, Sun H, Lu F, Zhang H
Molecular and cellular biology 2014 Jan;34(2):158-79

Hepatitis B virus X protein upregulates Lin28A/Lin28B through Sp-1/c-Myc to enhance the proliferation of hepatoma cells.

You X, Liu F, Zhang T, Lv N, Liu Q, Shan C, Du Y, Kong G, Wang T, Ye L, Zhang X
Oncogene 2014 Jan 23;33(4):449-60

Increased expression of Lin28B associates with poor prognosis in patients with oral squamous cell carcinoma.

Wu T, Jia J, Xiong X, He H, Bu L, Zhao Z, Huang C, Zhang W
PloS one 2013;8(12):e83869

The relationship between Lin28 and the chemotherapy response of gastric cancer.

Teng RY, Zhou JC, Jiang ZN, Xu CY, Li ZD, Wang QC, Xu CP, Guo JF, Shen JG, Wang LB
OncoTargets and therapy 2013;6:1341-5

Pluripotent stem cell protein Sox2 confers sensitivity to LSD1 inhibition in cancer cells.

Zhang X, Lu F, Wang J, Yin F, Xu Z, Qi D, Wu X, Cao Y, Liang W, Liu Y, Sun H, Ye T, Zhang H
Cell reports 2013 Oct 31;5(2):445-57

Lin28a is a putative factor in regulating cancer stem cell-like properties in side population cells of oral squamous cell carcinoma.

Hayashi S, Tanaka J, Okada S, Isobe T, Yamamoto G, Yasuhara R, Irie T, Akiyama C, Kohno Y, Tachikawa T, Mishima K
Experimental cell research 2013 May 1;319(8):1220-8

Implication of the SMN complex in the biogenesis and steady state level of the signal recognition particle.

Piazzon N, Schlotter F, Lefebvre S, Dodré M, Méreau A, Soret J, Besse A, Barkats M, Bordonné R, Branlant C, Massenet S
Nucleic acids research 2013 Jan;41(2):1255-72

SOX2-LIN28/let-7 pathway regulates proliferation and neurogenesis in neural precursors.

Cimadamore F, Amador-Arjona A, Chen C, Huang CT, Terskikh AV
Proceedings of the National Academy of Sciences of the United States of America 2013 Aug 6;110(32):E3017-26

In vivo generation of neural tumors from neoplastic pluripotent stem cells models early human pediatric brain tumor formation.

Werbowetski-Ogilvie TE, Morrison LC, Fiebig-Comyn A, Bhatia M
Stem cells (Dayton, Ohio) 2012 Mar;30(3):392-404

High expression of Lin28 is associated with tumour aggressiveness and poor prognosis of patients in oesophagus cancer.

Hamano R, Miyata H, Yamasaki M, Sugimura K, Tanaka K, Kurokawa Y, Nakajima K, Takiguchi S, Fujiwara Y, Mori M, Doki Y
British journal of cancer 2012 Apr 10;106(8):1415-23

RNA-binding protein LIN28 is a sensitive marker of ovarian primitive germ cell tumours.

Xue D, Peng Y, Wang F, Allan RW, Cao D
Histopathology 2011 Sep;59(3):452-9

Immunohistochemical expression of embryonic stem cell markers in malignant rhabdoid tumors.

Deisch J, Raisanen J, Rakheja D
Pediatric and developmental pathology : the official journal of the Society for Pediatric Pathology and the Paediatric Pathology Society 2011 Sep-Oct;14(5):353-9

RNA-binding protein LIN28 is a marker for testicular germ cell tumors.

Cao D, Allan RW, Cheng L, Peng Y, Guo CC, Dahiya N, Akhi S, Li J
Human pathology 2011 May;42(5):710-8

RNA-binding protein LIN28 is a marker for primary extragonadal germ cell tumors: an immunohistochemical study of 131 cases.

Cao D, Liu A, Wang F, Allan RW, Mei K, Peng Y, Du J, Guo S, Abel TW, Lane Z, Ma J, Rodriguez M, Akhi S, Dehiya N, Li J
Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc 2011 Feb;24(2):288-96

A nonviral minicircle vector for deriving human iPS cells.

Jia F, Wilson KD, Sun N, Gupta DM, Huang M, Li Z, Panetta NJ, Chen ZY, Robbins RC, Kay MA, Longaker MT, Wu JC
Nature methods 2010 Mar;7(3):197-9

Lin28a transgenic mice manifest size and puberty phenotypes identified in human genetic association studies.

Zhu H, Shah S, Shyh-Chang N, Shinoda G, Einhorn WS, Viswanathan SR, Takeuchi A, Grasemann C, Rinn JL, Lopez MF, Hirschhorn JN, Palmert MR, Daley GQ
Nature genetics 2010 Jul;42(7):626-30

Lin28 promotes transformation and is associated with advanced human malignancies.

Viswanathan SR, Powers JT, Einhorn W, Hoshida Y, Ng TL, Toffanin S, O'Sullivan M, Lu J, Phillips LA, Lockhart VL, Shah SP, Tanwar PS, Mermel CH, Beroukhim R, Azam M, Teixeira J, Meyerson M, Hughes TP, Llovet JM, Radich J, Mullighan CG, Golub TR, Sorensen PH, Daley GQ
Nature genetics 2009 Jul;41(7):843-8

A role for Lin28 in primordial germ-cell development and germ-cell malignancy.

West JA, Viswanathan SR, Yabuuchi A, Cunniff K, Takeuchi A, Park IH, Sero JE, Zhu H, Perez-Atayde A, Frazier AL, Surani MA, Daley GQ
Nature 2009 Aug 13;460(7257):909-13

No comments: Submit comment

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: K-562 cells were subjected to SDS PAGE followed by western blot with 11724-1-AP(LIN28 antibody) at dilution of 1:300
Sample type: cell line

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: Confocal immunofluorescent analysis of human embronic stem cells with 11724-1-AP at dilution of 1:400. The PE shows staining with 11724-1-AP/PE. The DAPI shows nuclear staining by DAPI. The MERGE is the merge of PE and DAPI. (20X)
Sample type: cell line

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: Confocal immunofluorescent analysis of human embronic stem cells with 11724-1-AP at dilution of 1:200. The PE shows staining with 11724-1-AP/PE. The DAPI shows nuclear staining by DAPI. The MERGE is the merge of PE and DAPI.(10X)
Sample type: cell line

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: IP Result of anti-LIN28 (IP:11724-1-AP, 3ug; Detection:11724-1-AP 1:500) with K-562 cells lysate 1000ug.
Sample type: cell line

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: Immunohistochemical of paraffin-embedded human prostate cancer using 11724-1-AP(LIN28 antibody) at dilution of 1:50 (under 40x lens)
Sample type: tissue