GTX628138

antibody from GeneTex

Targeting: LIN28A CSDD1, FLJ12457, LIN-28, LIN28, ZCCHC1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

GTX628138

Provider

GeneTex

Product name

Lin28A antibody [GT717]

Antibody type

Monoclonal

Reactivity

Human, Mouse

Host

Mouse

References

Submitted references

Over-expression of cofilin-1 suppressed growth and invasion of cancer cells is associated with up-regulation of let-7 microRNA.

Tsai CH, Lin LT, Wang CY, Chiu YW, Chou YT, Chiu SJ, Wang HE, Liu RS, Wu CY, Chan PC, Yang MH, Chiou SH, Liao MJ, Lee YJ
Biochimica et biophysica acta 2015 May;1852(5):851-61

Western blot

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Lin28A antibody [GT717] detects LIN28 protein by Western blot analysis.A. 30 £gg human ESC whole cell lysate/extract12 % SDS-PAGELin28A antibody [GT717] (GTX628138) dilution: 1:1000

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Sample (30 ug of whole cell lysate) A: NT2D1 12% SDS PAGE GTX628138 diluted at 1:1000

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Sample (30 ug of whole cell lysate) A: Non-transfected HeLa lysates B: LIN28 transfected HeLa lysates 12% SDS PAGE GTX628138 diluted at 1:1000

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Mouse tissue extract (50 £gg) was separated by 12% SDS-PAGE, and the membrane was blotted with Lin28A antibody [GT717] (GTX628138) diluted at 1:500. The signal was developed with Trident ECL plus-Enhanced.

Immunocytochemistry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

LIN28 antibody detects LIN28 protein by immunofluorescent analysis. Sample: human embryonic stem cell were fixed in 4% paraformaldehyde for 15 min. Green: LIN28 protein stained by LIN28 antibody (GTX628138) diluted at 1:200. Blue: Hoechst 33342 staining. Scale bar = 10 £gm.

Immunohistochemistry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Lin28A antibody detects Lin28A protein at cytosol on U87 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded U87 xenograft. Lin28A antibody (GTX628138) dilution: 1:100.

Flow cytometry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Lin28A antibody [GT717] detects Lin28A protein by flow cytomertry analysis. Sample: Human embryonic stem cells Black: Isotype control dilution: 1:50 Green: Lin28A antibody [GT717] dilution: 1:50