Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- AF1444 - Provider product page
- Provider
- R&D Systems
- Product name
- Mouse Angiopoietin-like Protein 2/ ANGPTL2 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects mouse Angiopoietin-like Protein 2/ANGPTL2 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant mouse (rm) ANGPTL6 and rmANGPTL1 is observed.
- Reactivity
- Mouse
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
Q9R045
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Cachexia-associated adipose loss induced by tumor-secreted leukemia inhibitory factor is counterbalanced by decreased leptin.
Angiopoietin-like protein 2 mediates endotoxin-induced acute inflammation in the eye.
Arora GK, Gupta A, Narayanan S, Guo T, Iyengar P, Infante RE
JCI insight 2018 Jul 26;3(14)
JCI insight 2018 Jul 26;3(14)
Angiopoietin-like protein 2 mediates endotoxin-induced acute inflammation in the eye.
Kanda A, Noda K, Oike Y, Ishida S
Laboratory investigation; a journal of technical methods and pathology 2012 Nov;92(11):1553-63
Laboratory investigation; a journal of technical methods and pathology 2012 Nov;92(11):1553-63
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Mouse Angiopoietin-like Protein 2/ANGPTL2 by Western Blot. Western blot shows lysates of mouse uterus tissue and MEF mouse embryonic feeder cells. PVDF membrane was probed with 2 µg/mL of Goat Anti-Mouse Angiopoietin-like Protein 2/ANGPTL2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1444) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Angiopoietin-like Protein 2/ANGPTL2 at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.