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Flow cytometryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Flow cytometry [1]
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- Product number
- MAB52351 - Provider product page
- Provider
- R&D Systems
- Product name
- Mouse Semaphorin 4D/CD100 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects mouse Semaphorin 4D/CD100 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human Semaphorin 3A, 3B, 3C, 3D, 3E, 3F, 4A, 4B, 4C, 4D, 4G, 5A, 5B, 6A, 6B, 6C, 6D, or 7A is observed.
- Reactivity
- Mouse
- Host
- Rat
- Conjugate
- Unconjugated
- Antigen sequence
NP_038688- Isotype
- IgG
- Antibody clone number
- 547412
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
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Supportive validation
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- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Semaphorin 4D/CD100 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Mouse Semaphorin 4D/CD100 Monoclonal Antibody (Catalog # MAB52351, filled histogram) or isotype control antibody (Catalog # MAB0061, open histogram), followed by Phycoerythrin-conjugated Anti-Rat IgG F(ab')2 Secondary Antibody (Catalog # F0105B).
