TA809844

antibody from OriGene

Targeting: SEMA4D C9orf164, CD100, coll-4, FLJ39737, SEMAJ

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

TA809844

Provider

OriGene

Product name

SEMA4D mouse monoclonal antibody,clone OTI6G1

Antibody type

Monoclonal

Description

SEMA4D mouse monoclonal antibody,clone OTI6G1

Host

Mouse

Conjugate

Unconjugated

Epitope

SEMA4D

Isotype

IgG

Antibody clone number

OTI6G1

Vial size

100 µl

Concentration

1.00mg/ml

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SEMA4D (RC208521, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SEMA4D.(1:2000)

Validation comment

WB

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Western blot analysis of extracts (35ug) from 2 different cell lines by using anti-SEMA4D monoclonal antibody .(1:500)

Validation comment

WB

Immunohistochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-SEMA4D mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA809844)(1:500)

Validation comment

IHC