14-1009-82

antibody from Invitrogen Antibodies
Targeting: SEMA4D C9orf164, CD100, coll-4, FLJ39737, SEMAJ
Provider product page for 14-1009-82
ELISA
Immunoprecipitation
Flow cytometry

Antibody data

Product number
14-1009-82 - Provider product page
Provider
Invitrogen Antibodies
Product name
CD100 (SEMA4D) Monoclonal Antibody (eBio133-1C6 (133-1C6)), eBioscience™
Antibody type
Monoclonal
Antigen
Other
Description
Description: The eBio133-1C6 monoclonal antibody reacts with human CD100, a member of the class IV semaphorin family also called sema4D. CD100 promotes angiogenesis, invasive growth, proliferation, and anti-apoptosis of cancer cells in vitro. Furthermore CD100 is a costimulatory molecule in adaptive immune responses by switching off negative signalling from CD72. In addition to CD72 another ligand is Plexin-B1. It has been shown that higher expression levels of CD100 are correlated with poor survival in soft tissue sarcoma patients. Expression is found in both hematopoietic and non-hematopoietic cells.CD100 is expressed on resting T cells and NK cells and B cell. CD100 can also exist as a soluble form. Applications Reported: This eBio133-1C6 (133-1C6) antibody has been reported for use in flow cytometric analysis, immunoprecipitation, and ELISA. Applications Tested: This eBio133-1C6 (133-1C6) antibody has been tested by flow cytometric analysis of human peripheral blood cells. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 µm post-manufacturing filtered.
Reactivity
Human
Host
Mouse
Isotype
IgM
Antibody clone number
eBio133-1C6 (133-1C6)
Vial size
100 µg
Concentration
0.5 mg/mL
Storage
4°C
SEMA4D protein structure - 14-1009-82 shown in red.