53-1009-42
antibody from Invitrogen Antibodies
Targeting: SEMA4D
C9orf164, CD100, coll-4, FLJ39737, SEMAJ
Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Flow cytometry [1]
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Validation data
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- Product number
- 53-1009-42 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CD100 (SEMA4D) Monoclonal Antibody (eBio133-1C6 (133-1C6)), Alexa Fluor™ 488, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: The eBio133-1C6 monoclonal antibody reacts with human CD100, a member of the class IV semaphorin family also called sema4D. CD100 promotes angiogenesis, invasive growth, proliferation, and anti-apoptosis of cancer cells in vitro. Furthermore CD100 is a costimulatory molecule in adaptive immune responses by switching off negative signalling from CD72. In addition to CD72 another ligand is Plexin-B1. It has been shown that higher expression levels of CD100 are correlated with poor survival in soft tissue sarcoma patients. Expression is found in both hematopoietic and non-hematopoietic cells.CD100 is expressed on resting T cells and NK cells and B cell. CD100 can also exist as a soluble form. Applications Reported: This eBio133-1C6 (133-1C6) antibody has been reported for use in flow cytometric analysis. Applications Tested: This eBio133-1C6 (133-1C6) antibody has been pre-titrated and tested by flow cytometric of human peripheral blood cells. This can be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser. Filtration: 0.2 µm post-manufacturing filtered.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Green dye
- Isotype
- IgM
- Antibody clone number
- eBio133-1C6 (133-1C6)
- Vial size
- 100 Tests
- Concentration
- 5 µL/Test
- Storage
- 4° C, store in dark, DO NOT FREEZE!
Submitted references Tax and Semaphorin 4D Released from Lymphocytes Infected with Human Lymphotropic Virus Type 1 and Their Effect on Neurite Growth.
Quintremil S, Alberti C, Rivera M, Medina F, Puente J, Cartier L, RamÃrez E, Tanaka Y, Valenzuela MA
AIDS research and human retroviruses 2016 Jan;32(1):68-79
AIDS research and human retroviruses 2016 Jan;32(1):68-79
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Staining of normal human peripheral blood cells with staining buffer (autofluorescence) (open histogram) or Anti-Human CD100 Alexa Fluor® 488 (filled histogram). Cells in the lymphocyte gate were used for analysis.
- Conjugate
- Green dye