NBP2-61663
antibody from Novus Biologicals
Targeting: SEMA4D
C9orf164, CD100, coll-4, FLJ39737, SEMAJ
Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- ELISA [1]
- Immunohistochemistry [2]
- Flow cytometry [1]
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Validation data
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- Product number
- NBP2-61663 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal Semaphorin 4D/CD100 Antibody
- Antibody type
- Monoclonal
- Description
- Protein G purified.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Semaphorin 4D/CD100 Antibody (5H6E3) [NBP2-61663] - Analysis using CD100 mAb against HEK293 (1) and CD100 (AA: extra 590-734)-hIgGFc transfected HEK293 (2) cell lysate.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Semaphorin 4D/CD100 Antibody (5H6E3) [NBP2-61663] - Analysis using CD100 mAb against human CD100 (AA: extra 590-734) recombinant protein. (Expected MW is 43.2 kDa)
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- ELISA: Semaphorin 4D/CD100 Antibody (5H6E3) [NBP2-61663] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: Semaphorin 4D/CD100 Antibody (5H6E3) [NBP2-61663] - Analysis of paraffin-embedded cervical cancer tissues using CD100 mouse mAb with DAB staining.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: Semaphorin 4D/CD100 Antibody (5H6E3) [NBP2-61663] - Analysis of paraffin-embedded bladder cancer tissues using CD100 mouse mAb with DAB staining.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: Semaphorin 4D/CD100 Antibody (5H6E3) [NBP2-61663] - Analysis of Ramos cells using CD100 mouse mAb (green) and negative control (red).