GTX103949

antibody from GeneTex

Targeting: IP6K1 IHPK1, KIAA0263

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

GTX103949

Provider

GeneTex

Proper citation

GeneTex Cat#GTX103949, RRID:AB_1950559

Product name

IP6K1 antibody

Antibody type

Polyclonal

Reactivity

Human, Mouse

Host

Rabbit

References

Submitted references

Global IP6K1 deletion enhances temperature modulated energy expenditure which reduces carbohydrate and fat induced weight gain.

Zhu Q, Ghoshal S, Tyagi R, Chakraborty A
Molecular metabolism 2017 Jan;6(1):73-85

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IP6K1 Reduces Mesenchymal Stem/Stromal Cell Fitness and Potentiates High Fat Diet-Induced Skeletal Involution.

Boregowda SV, Ghoshal S, Booker CN, Krishnappa V, Chakraborty A, Phinney DG
Stem cells (Dayton, Ohio) 2017 Aug;35(8):1973-1983

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Inositol hexakisphosphate kinase-1 interacts with perilipin1 to modulate lipolysis.

Ghoshal S, Tyagi R, Zhu Q, Chakraborty A
The international journal of biochemistry & cell biology 2016 Sep;78:149-155

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Adipocyte-specific deletion of Ip6k1 reduces diet-induced obesity by enhancing AMPK-mediated thermogenesis.

Zhu Q, Ghoshal S, Rodrigues A, Gao S, Asterian A, Kamenecka TM, Barrow JC, Chakraborty A
The Journal of clinical investigation 2016 Nov 1;126(11):4273-4288

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Inositol Hexakisphosphate Kinase 1 (IP6K1) Regulates Inositol Synthesis in Mammalian Cells.

Yu W, Ye C, Greenberg ML
The Journal of biological chemistry 2016 May 13;291(20):10437-44

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Inositol pyrophosphates promote tumor growth and metastasis by antagonizing liver kinase B1.

Rao F, Xu J, Fu C, Cha JY, Gadalla MM, Xu R, Barrow JC, Snyder SH
Proceedings of the National Academy of Sciences of the United States of America 2015 Feb 10;112(6):1773-8

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Inositol hexakisphosphate kinase-1 mediates assembly/disassembly of the CRL4-signalosome complex to regulate DNA repair and cell death.

Rao F, Xu J, Khan AB, Gadalla MM, Cha JY, Xu R, Tyagi R, Dang Y, Chakraborty A, Snyder SH
Proceedings of the National Academy of Sciences of the United States of America 2014 Nov 11;111(45):16005-10

Western blot

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

IP6K1 antibody detects IP6K1 protein by western blot analysis.A. 30 ?g 293T whole cell lysate/extractB. 30 ?g whole cell lysate/extract of human IHPK1-transfected 293T cells10% SDS-PAGEIP6K1 antibody (GTX103949) dilution: 1:5000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

IP6K1 antibody detects IP6K1 protein by western blot analysis.A. 30 ?g Jurkat whole cell lysate/extract B. 30 ?g Raji whole cell lysate/extractC. 30 ?g THP-1 whole cell lysate/extractD. 30 ?g NCI-H929 whole cell lysate/extract10% SDS-PAGEIP6K1 antibody (GTX103949) dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Various whole cell extracts (30 ?g) were separated by 10% SDS-PAGE, and the membrane was blotted with IP6K1 antibody (GTX103949) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Immunocytochemistry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using IP6K1(GTX103949) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.

Immunohistochemistry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded Ca922 Xenograft, using IP6K1(GTX103949) antibody at 1:500 dilution.