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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [5]
- Immunocytochemistry [2]
- Immunohistochemistry [1]
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- Product number
- PA5-21531 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- IP6K1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: Jurkat, Raji, NCI-H929, human IHPK1-transfected 293T. Predicted reactivity: Mouse (97%), Rat (96%), Rhesus Monkey (99%), Bovine (97%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.22 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of IP6K1 using A) 30 µg Jurkat whole cell lysate (B) 30 µg Raji whole cell lysate (C) 30 µg THP-1 whole cell lysate and D) 30 µg NCI-H929 whole cell lysate. Samples were loaded onto a 10% SDS-PAGE gel and probed with an IP6K1 polyclonal antibody (Product # PA5-21531) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of IP6K1 using A) 30 µg 293T whole cell lysate and B) 30 µg whole cell lysate of human IHPK1-transfected 293T cells. Samples were loaded onto a 10% SDS-PAGE gel and probed with an IP6K1 polyclonal antibody (Product # PA5-21531) at a dilution of 1:5000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of IP6K1 was performed by separating 30 µg of various whole cell extracts by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a IP6K1 Polyclonal Antibody (Product # PA5-21531) at a dilution of 1:1000 and a HRP-conjugated anti-rabbit IgG secondary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of IP6K1 was performed by separating 30 µg of various whole cell extracts by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a IP6K1 Polyclonal Antibody (Product # PA5-21531) at a dilution of 1:1000 and a HRP-conjugated anti-rabbit IgG secondary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using IP6K1 Polyclonal Antibody (Product # PA5-21531). Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with IP6K1 Polyclonal Antibody (Product # PA5-21531) diluted at 1:5,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of IP6K1 in paraformaldehyde-fixed HeLa cells using an IP6K1 polyclonal antibody (Product # PA5-21531) (Green) at a 1:500 dilution. Alpha-tubulin filaments were labeled with Product # PA5-29281 (Red) at a 1:2000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using IP6K1 antibody (Product # PA5-21531) (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Product # MA1-25054) (Red) at 1:2,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded Ca922 Xenograft, using IP6K1 (Product # PA5-21531) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
