PA5-99691

antibody from Invitrogen Antibodies

Targeting: POLR3B C128, FLJ10388, RPC2

Western blot (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-99691

Provider

Invitrogen Antibodies

Product name

POLR3B Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Antibody detects endogenous levels of total POLR3B.

Reactivity

Human, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1 mg/mL

Storage

-20°C

References

Submitted references

Truncated PARP1 mediates ADP-ribosylation of RNA polymerase III for apoptosis.

Chen Q, Ma K, Liu X, Chen SH, Li P, Yu Y, Leung AKL, Yu X
Cell discovery 2022 Jan 18;8(1):3

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of POLR3B in LOVO. Samples were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 10% serum (45 min at 25°C) incubated with POLR3B polyclonal antibody (Product # PA5-99691) using a dilution of 1:200 (1 hr, 37°C), and followed by goat anti-rabbit IgG Alexa Fluor 594 at a dilution of 1:600.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Fig. 2 The BRCT domain of tPARP1 interacts with the Pol III complex. a U2OS-PARP1 knockout cells were transfected with the myc-empty vector (EV), myc-POLR3A, myc-POLR3B, or myc-POLR3F together with HA-mtPARP1, followed by IP and western blotting with the indicated antibodies. b mtPARP1 or full-length mPARP1 was ectopically expressed in U2OS-PARP1 knockout cells. IP was performed with IgG as a negative control. c U2OS-PARP1 knockout cells were transfected with the myc-empty vector (EV), myc-POLR3A, myc-POLR3B, or myc-POLR3F together with HA-mTARG1, followed by IP and western blotting with the indicated antibodies. d U2OS-PARP1 knockout cells were transfected with the HA-tagged truncation mutants of mtPARP1 together with myc-POLR3A (upper panel), myc-POLR3B (middle panel), or myc-POLR3F (lower panel). e U2OS-PARP1 knockout cells were transfected with the indicated combinations of HA-tagged mtPARP1 or mtPARP1 F473A mutant together with myc-POLR3A (upper panel), myc-POLR3B (middle panel), or myc-POLR3F (lower panel).