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Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Flow cytometry [1]
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- Product number
- RQ4536 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- TMEM16A Antibody / DOG1 / ANO1
- Antibody type
- Polyclonal
- Description
- This highly specific TMEM16A antibody is suitable for use in Western blot/Immunohistochemistry/Flow cytometry applications with human samples.
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Vial size
- 100 ug
- Concentration
- 0.5mg/ml if reconstituted with 0.2ml sterile DI water
- Storage
- After reconstitution, the TMEM16A antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of human 1) HeLa, 2) HepG2, 3) A549, 4) PANC-1, 5) SK-OV-3, 6) SGC-7901 and 7) COLO-320 lysate with TMEM16A antibody at 0.5ug/ml. Expected molecular weight 114~130 kDa depending on glycosylation level.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Flow cytometry testing of fixed and permeabilized human A431 cells with TMEM16A antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= TMEM16A antibody.
