HPA032148

antibody from Atlas Antibodies

Targeting: ANO1 DOG1, FLJ10261, ORAOV2, TAOS2, TMEM16A

Immunohistochemistry (Enhanced validation)

Antibody Data

Product number

HPA032148

Provider

Atlas Antibodies

Proper citation

Atlas Antibodies Cat#HPA032148, RRID:AB_2674180

Product name

Anti-ANO1

Antibody type

Polyclonal

Reactivity

Human

Host

Rabbit

Conjugate

Unconjugated

Antigen sequence

RVNEKYSTLPAEDRSVHIINICAIEDIGYLPSEGT
LLNSLSVDPDAECKYGLYFRDGRRKVDYILVYHHK
RPSGNRTLVRRVQHSDTPSGARSVKQDHP


Isotype

IgG

Vial size

100 µl

Storage

Store at +4°C for short term storage. Long time storage is recommended at -20°C.

Immunohistochemistry

Enhanced validation

Submitted by

Atlas Antibodies (provider)

Enhanced method

Orthogonal validation

Main image

Experimental details

Immunohistochemistry analysis in human seminal vesicle and lymph node tissues using Anti-ANO1 antibody. Corresponding ANO1 RNA-seq data are presented for the same tissues.

Sample type

HUMAN

Orthogonal method

Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.

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Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human gall bladder shows strong cytoplasmic and membrane positivity in glandular cells.

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human seminal vesicle shows high expression.

Sample type

HUMAN

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human lymph node shows low expression as expected.

Sample type

HUMAN