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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [12]
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- Validations
- Western blot [1]
- Immunohistochemistry [2]
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- Product number
- AP1802b - Provider product page
- Provider
- Abcepta
- Proper citation
- Abgent Cat#AP1802b, RRID:AB_2137731
- Product name
- LC3 Antibody (APG8B) (C-term)
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Purified Rabbit Polyclonal Antibody (Pab)
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 400 µl
- Concentration
- 2 mg/ml
- Storage
- Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Submitted references p65/RelA-Ser529 NF-κB subunit phosphorylation induces autophagic astroglial death (Clasmatodendrosis) following status epilepticus.
p62, Ref(2)P and ubiquitinated proteins are conserved markers of neuronal aging, aggregate formation and progressive autophagic defects.
Integrating multiple aspects of mitochondrial dynamics in neurons: age-related differences and dynamic changes in a chronic rotenone model.
Knockdown of the DNA-dependent protein kinase catalytic subunit radiosensitizes glioma-initiating cells by inducing autophagy.
Parkinson's disease involves autophagy and abnormal distribution of cathepsin L.
Functional evidence implicating a novel TOR1A mutation in idiopathic, late-onset focal dystonia.
Helicobacter pylori impairs murine dendritic cell responses to infection.
Autophagy is a protective mechanism in normal cartilage, and its aging-related loss is linked with cell death and osteoarthritis.
A novel quantitative flow cytometry-based assay for autophagy.
Acquired cisplatin resistance in human lung adenocarcinoma cells is associated with enhanced autophagy.
Therapeutic potential of a synthetic lethal interaction between the MYC proto-oncogene and inhibition of aurora-B kinase.
Regulation of the autophagy protein LC3 by phosphorylation.
Ryu HJ, Kim JE, Yeo SI, Kang TC
Cellular and molecular neurobiology 2011 Oct;31(7):1071-8
Cellular and molecular neurobiology 2011 Oct;31(7):1071-8
p62, Ref(2)P and ubiquitinated proteins are conserved markers of neuronal aging, aggregate formation and progressive autophagic defects.
Bartlett BJ, Isakson P, Lewerenz J, Sanchez H, Kotzebue RW, Cumming RC, Harris GL, Nezis IP, Schubert DR, Simonsen A, Finley KD
Autophagy 2011 Jun;7(6):572-83
Autophagy 2011 Jun;7(6):572-83
Integrating multiple aspects of mitochondrial dynamics in neurons: age-related differences and dynamic changes in a chronic rotenone model.
Arnold B, Cassady SJ, VanLaar VS, Berman SB
Neurobiology of disease 2011 Jan;41(1):189-200
Neurobiology of disease 2011 Jan;41(1):189-200
Knockdown of the DNA-dependent protein kinase catalytic subunit radiosensitizes glioma-initiating cells by inducing autophagy.
Zhuang W, Li B, Long L, Chen L, Huang Q, Liang ZQ
Brain research 2011 Jan 31;1371:7-15
Brain research 2011 Jan 31;1371:7-15
Parkinson's disease involves autophagy and abnormal distribution of cathepsin L.
Li L, Wang X, Fei X, Xia L, Qin Z, Liang Z
Neuroscience letters 2011 Feb 1;489(1):62-7
Neuroscience letters 2011 Feb 1;489(1):62-7
Functional evidence implicating a novel TOR1A mutation in idiopathic, late-onset focal dystonia.
Calakos N, Patel VD, Gottron M, Wang G, Tran-Viet KN, Brewington D, Beyer JL, Steffens DC, Krishnan RR, Züchner S
Journal of medical genetics 2010 Sep;47(9):646-50
Journal of medical genetics 2010 Sep;47(9):646-50
Helicobacter pylori impairs murine dendritic cell responses to infection.
Wang YH, Gorvel JP, Chu YT, Wu JJ, Lei HY
PloS one 2010 May 27;5(5):e10844
PloS one 2010 May 27;5(5):e10844
Autophagy is a protective mechanism in normal cartilage, and its aging-related loss is linked with cell death and osteoarthritis.
Caramés B, Taniguchi N, Otsuki S, Blanco FJ, Lotz M
Arthritis and rheumatism 2010 Mar;62(3):791-801
Arthritis and rheumatism 2010 Mar;62(3):791-801
A novel quantitative flow cytometry-based assay for autophagy.
Eng KE, Panas MD, Karlsson Hedestam GB, McInerney GM
Autophagy 2010 Jul;6(5):634-41
Autophagy 2010 Jul;6(5):634-41
Acquired cisplatin resistance in human lung adenocarcinoma cells is associated with enhanced autophagy.
Ren JH, He WS, Nong L, Zhu QY, Hu K, Zhang RG, Huang LL, Zhu F, Wu G
Cancer biotherapy & radiopharmaceuticals 2010 Feb;25(1):75-80
Cancer biotherapy & radiopharmaceuticals 2010 Feb;25(1):75-80
Therapeutic potential of a synthetic lethal interaction between the MYC proto-oncogene and inhibition of aurora-B kinase.
Yang D, Liu H, Goga A, Kim S, Yuneva M, Bishop JM
Proceedings of the National Academy of Sciences of the United States of America 2010 Aug 3;107(31):13836-41
Proceedings of the National Academy of Sciences of the United States of America 2010 Aug 3;107(31):13836-41
Regulation of the autophagy protein LC3 by phosphorylation.
Cherra SJ 3rd, Kulich SM, Uechi G, Balasubramani M, Mountzouris J, Day BW, Chu CT
The Journal of cell biology 2010 Aug 23;190(4):533-9
The Journal of cell biology 2010 Aug 23;190(4):533-9
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Supportive validation
- Submitted by
- Abcepta (provider)
- Main image
- Experimental details
- Western blot analysis of anti-LC3 (APG8b) Pab (Cat. #AP1802b) in 293 cell line lysates transiently transfected with the LC3 (APG8b) gene (2ug/lane). LC3 (APG8b) (arrow) was detected using the purified Pab.
- Primary Ab dilution
- 1:1000
Supportive validation
- Submitted by
- Abcepta (provider)
- Main image
- Experimental details
- "Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma."
- Primary Ab dilution
- 1:50~100
- Submitted by
- Abcepta (provider)
- Main image
- Experimental details
- "Formalin-fixed and paraffin-embedded human testis tissue reacted with Autophagy LC3 antibody (APG8b) (C-term) (Cat.#AP1802b), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated."
- Primary Ab dilution
- 1:50~100
