Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
- Flow cytometry [1]
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Validation data
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- Product number
- F46116 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- LC3II Antibody
- Antibody type
- Polyclonal
- Antigen
- A portion of amino acids 74-106 from the human protein was used as the immunogen for this LC3B / LC3II antibody.
- Description
- Antigen affinity purified antibody
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Conjugate
- Unconjugated
- Vial size
- 80 µl, 400 µl
- Storage
- Aliquot the LC3II antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot analysis of LC3II antibody and MCF-7 lysate
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- LC3II antibody western blot analysis in mouse cerebellum tissue lysate
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- LC3II antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- LC3II antibody flow cytometric analysis of MCF-7 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.