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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [3]
- Immunohistochemistry [1]
- Flow cytometry [1]
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Validation data
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- Product number
- PA5-30598 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- LC3B Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Recommended positive controls: U87-MG, Mouse brain, Rat brain.
- Concentration
- 0.37 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using LC3B Polyclonal Antibody (Product # PA5-30598). Sample (50 µg of whole cell lysate). Lane A: Rat brain. 12% SDS PAGE. LC3B Polyclonal Antibody (Product # PA5-30598) diluted at 1:1,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using LC3B Polyclonal Antibody (Product # PA5-30598). Sample (50 µg of whole cell lysate). Lane A: mouse brain . 15% SDS PAGE. LC3B Polyclonal Antibody (Product # PA5-30598) diluted at 1:1,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using LC3B Polyclonal Antibody (Product # PA5-30598). Sample (30 µg of whole cell lysate). Lane A: U87-MG . 15% SDS PAGE. LC3B Polyclonal Antibody (Product # PA5-30598) diluted at 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human Breast carcinoma, using LC3B (Product # PA5-30598) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- LC3B Polyclonal Antibody (Product # PA5-30598) detects LC3B protein by flow cytometry analysis. Sample: HeLa cell fixed in 4% paraformaldehyde at 4°C for 5 min. Brown: Unlabelled sample was also used as a control. Blue: LC3B Polyclonal Antibody (Product # PA5-30598) dilution: 1:100. Acquisition of >20,000 events were collected using Argon ion laser (488nm) and 525/30 bandpass filter.
