PA5-32254

antibody from Invitrogen Antibodies

Targeting: MAP1LC3B ATG8F

Western blot

Antibody data

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Validation data

Product number: PA5-32254 - Provider product page
Provider: Invitrogen Antibodies
Product name: LC3B Polyclonal Antibody
Antibody type: Polyclonal
Antigen: Synthetic peptide
Description: Recommended positive controls: NT2D1, PC-3, U87-MG, SK-N-SH, mouse brain, Rat brain, HepG2 (untreated), HepG2 (3 µM Thapsigargin treatment for 12 hr), HepG2 (3 µM Thapsigargin treatment for 16hr), HepG2 (3 µM Thapsigargin treatment for 24hr), Huh7 (un-infected), Huh7 (HCV-infected), HeLa, HeLa (50 µM Chloroquine treatment for 24 hr). Predicted reactivity: Zebrafish (100%), Japanese Medaka (100%), Pig (100%), Rhesus Monkey (100%), Chimpanzee (100%), Bovine (100%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Reactivity: Human, Mouse, Rat, Porcine
Host: Rabbit
Isotype: IgG
Vial size: 100 µL
Concentration: 0.34 mg/mL
Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

MAP1LC3B protein structure - PA5-32254 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescent analysis of LC3B1+LC3B2 showing staining in the autophagosome of Hep G2 cells. Hep G2 cells mock (left) and treated with 3µM Thapsigargin for 12 hrs (right) were fixed in ice-cold MeOH for 10 min and permeabilized with ice-cold acetone for 1 min and stained using a LC3B1+LC3B2 polyclonal antibody (Product # PA5-32254) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10µm.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescent analysis of LC3B1+LC3B2 showing staining in the autophagosome of HeLa cells. HeLa cells mock (left) and treated with 50µM Chloroquine for 24 hr (right) were fixed in 4% paraformaldehyde at RT for 15 min and stained using a LC3B1+LC3B2 polyclonal antibody (Product # PA5-32254) diluted at 1:1000. Red: Phalloidin, a F-actin marker.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunocytochemistry-Immunofluorescence analysis of LC3B in HeLa cells mock (left) and in HeLa cells treated with 50 µM Chloroquine for 24 hr (right) fixed with 4% paraformaldehyde at RT for 15 min. Green: LC3B Polyclonal Antibody (Product # PA5-32254) diluted at 1:2,000. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody. Blue: Hoechst 33342 staining.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunocytochemistry-Immunofluorescence analysis of LC3B was performed in HeLa cells fixed in 4% paraformaldehyde at RT for 15 min. Green: LC3B Polyclonal Antibody (Product # PA5-32254) diluted at 1:200. Red: phalloidin, a cytoskeleton marker. Blue: Hoechst 33342 staining. Scale bar = 10 µm.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunocytochemistry-Immunofluorescence analysis of LC3B in HeLa cells mock (left) and in HeLa cells treated with 50 µM Chloroquine for 24 hr (right) fixed with 4% paraformaldehyde at RT for 15 min. Green: LC3B Polyclonal Antibody (Product # PA5-32254) diluted at 1:2,000. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody. Blue: Hoechst 33342 staining.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunocytochemistry-Immunofluorescence analysis of LC3B was performed in HeLa cells fixed in 4% paraformaldehyde at RT for 15 min. Green: LC3B Polyclonal Antibody (Product # PA5-32254) diluted at 1:200. Red: phalloidin, a cytoskeleton marker. Blue: Hoechst 33342 staining. Scale bar = 10 µm.