Antibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Immunocytochemistry [1]
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Validation data
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- Product number
- ALX-803-080-C100 - Provider product page
- Provider
- Enzo Life Sciences
- Proper citation
- Enzo Life Sciences Cat#ALX-803-080-C100, RRID:AB_2051773
- Product name
- LC3B monoclonal antibody (5F10)
- Antibody type
- Monoclonal
- Antigen
- Synthetic peptide
- Description
- Purified from serum-free cell culture supernatant by subsequent ultrafiltration and size exclusion chromatography.
- Reactivity
- Human, Mouse, Rat, Canine, Hamster
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 5F10
- Vial size
- 100 μg
- Storage
- -20°C
- Handling
- Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and freeze in liquid nitrogen.
Submitted references The nuclear cofactor DOR regulates autophagy in mammalian and Drosophila cells.
Discovery of Atg5/Atg7-independent alternative macroautophagy.
Early endosomes and endosomal coatomer are required for autophagy.
Mauvezin C, Orpinell M, Francis VA, Mansilla F, Duran J, Ribas V, PalacĂn M, Boya P, Teleman AA, Zorzano A
EMBO reports 2010 Jan;11(1):37-44
EMBO reports 2010 Jan;11(1):37-44
Discovery of Atg5/Atg7-independent alternative macroautophagy.
Nishida Y, Arakawa S, Fujitani K, Yamaguchi H, Mizuta T, Kanaseki T, Komatsu M, Otsu K, Tsujimoto Y, Shimizu S
Nature 2009 Oct 1;461(7264):654-8
Nature 2009 Oct 1;461(7264):654-8
Early endosomes and endosomal coatomer are required for autophagy.
Razi M, Chan EY, Tooze SA
The Journal of cell biology 2009 Apr 20;185(2):305-21
The Journal of cell biology 2009 Apr 20;185(2):305-21
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Supportive validation
- Submitted by
- Enzo Life Sciences (provider)
- Main image
- Experimental details
- Endogenous LC3 punctae detected using MAb to LC3B (5F10) (Prod. No. ALX-803-080)Method: The majority of LC3 was diffusely localized in unstimulated COS-7 cells, wheres punctated signals of LC3 increase after induction of autophagy by vinblastin stimulation for 2h. Cells were fixed with paraformaldehyde followed by methanol treatment. Cells were permeabilized with 0.3% Triton X-100. (Image courtesy of I. Ciechomska and A. Tolkovsky, University of Cambridge, UK.)