14175-1-AP

antibody from Proteintech Group

Targeting: PPFIA1 LIP.1, LIPRIN

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Data presented on provider website)

Antibody Data

Product number

14175-1-AP

Provider

Proteintech Group

Proper citation

Proteintech Cat#14175-1-AP, RRID:AB_2171592

Product name

Liprin Alpha 1 antibody

Antibody type

Polyclonal

Description

KD/KO validated Liprin Alpha 1 antibody (Cat. #14175-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: FC, IF, IHC, IP, WB,ELISA.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Conjugate

Unconjugated

Isotype

IgG

Vial size

20ul, 150ul

References

Submitted references

Caskin2 is a novel talin- and Abi1-binding protein that promotes cell motility.

Wang W, Atherton P, Kreft M, Te Molder L, van der Poel S, Hoekman L, Celie P, Joosten RP, Fässler R, Perrakis A, Sonnenberg A
Journal of cell science 2024 May 1;137(9)

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Liprin-α1 contributes to oncogenic MAPK signaling by counteracting ERK activity.

Pehkonen H, Filippou A, Väänänen J, Lindfors I, Vänttinen M, Ianevski P, Mäkelä A, Munne P, Klefström J, Toppila-Salmi S, Grénman R, Hagström J, Mäkitie AA, Karhemo PR, Monni O
Molecular oncology 2024 Mar;18(3):662-676

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Dual specificity kinase DYRK3 regulates cell migration by influencing the stability of protrusions.

Ramella M, Ribolla LM, Surini S, Sala K, Tonoli D, Cioni JM, Rai AK, Pelkmans L, de Curtis I
iScience 2024 Apr 19;27(4):109440

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Interfering with the ERC1-LL5β interaction disrupts plasma membrane-Associated platforms and affects tumor cell motility.

Ribolla LM, Sala K, Tonoli D, Ramella M, Bracaglia L, Bonomo I, Gonnelli L, Lamarca A, Brindisi M, Pierattelli R, Provenzani A, de Curtis I
PloS one 2023;18(7):e0287670

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Liprin-α1 Expression in Tumor-Infiltrating Lymphocytes Associates with Improved Survival in Patients with HPV-Positive Oropharyngeal Squamous Cell Carcinoma.

Sjöblom A, Pehkonen H, Jouhi L, Monni O, Randén-Brady R, Karhemo PR, Tarkkanen J, Haglund C, Mattila P, Mäkitie A, Hagström J, Carpén T
Head and neck pathology 2023 Sep;17(3):647-657

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Clinical, neuroimaging and molecular characteristics of PPP2R5D-related neurodevelopmental disorders: an expanded series with functional characterisation and genotype-phenotype analysis.

Oyama N, Vaneynde P, Reynhout S, Pao EM, Timms A, Fan X, Foss K, Derua R, Janssens V, Chung W, Mirzaa GM
Journal of medical genetics 2023 May;60(5):511-522

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High PPFIA1 expression promotes cancer survival by suppressing CD8+ T cells in breast cancer: drug discovery and machine learning approach.

Chu J, Min KW, Kim DH, Son BK, Kim HS, Jung US, Kwon MJ, Do SI
Breast cancer (Tokyo, Japan) 2023 Mar;30(2):259-270

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The structural basis of the talin-KANK1 interaction that coordinates the actin and microtubule cytoskeletons at focal adhesions.

Li X, Goult BT, Ballestrem C, Zacharchenko T
Open biology 2023 Jun;13(6):230058

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Functional glycoproteomics by integrated network assembly and partitioning.

Griffin ME, Thompson JW, Xiao Y, Sweredoski MJ, Aksenfeld RB, Jensen EH, Koldobskaya Y, Schacht AL, Kim TD, Choudhry P, Lomenick B, Garbis SD, Moradian A, Hsieh-Wilson LC
bioRxiv : the preprint server for biology 2023 Jun 14;

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The CEP170B-KIF2A complex destabilizes microtubule minus ends to generate polarized microtubule network.

Guan C, Hua S, Jiang K
The EMBO journal 2023 Jun 1;42(11):e112953

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A functional interaction between liprin-α1 and B56γ regulatory subunit of protein phosphatase 2A supports tumor cell motility.

Ripamonti M, Lamarca A, Davey NE, Tonoli D, Surini S, de Curtis I
Communications biology 2022 Sep 28;5(1):1025

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Glucose-Dependent Insulin Secretion from β Cell Spheroids Is Enhanced by Embedding into Softer Alginate Hydrogels Functionalised with RGD Peptide.

Amin ML, Deng K, Tran HA, Singh R, Rnjak-Kovacina J, Thorn P
Bioengineering (Basel, Switzerland) 2022 Nov 23;9(12)

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Local activation of focal adhesion kinase orchestrates the positioning of presynaptic scaffold proteins and Ca(2+) signalling to control glucose-dependent insulin secretion.

Jevon D, Deng K, Hallahan N, Kumar K, Tong J, Gan WJ, Tran C, Bilek M, Thorn P
eLife 2022 May 13;11

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Enhanced structure and function of human pluripotent stem cell-derived beta-cells cultured on extracellular matrix.

Singh R, Cottle L, Loudovaris T, Xiao D, Yang P, Thomas HE, Kebede MA, Thorn P
Stem cells translational medicine 2021 Mar;10(3):492-505

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Structural basis of liprin-α-promoted LAR-RPTP clustering for modulation of phosphatase activity.

Xie X, Luo L, Liang M, Zhang W, Zhang T, Yu C, Wei Z
Nature communications 2020 Jan 10;11(1):169

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The ERC1 scaffold protein implicated in cell motility drives the assembly of a liquid phase.

Sala K, Corbetta A, Minici C, Tonoli D, Murray DH, Cammarota E, Ribolla L, Ramella M, Fesce R, Mazza D, Degano M, de Curtis I
Scientific reports 2019 Sep 19;9(1):13530

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Characterization of the polarized endothelial secretome.

Wei H, Sundararaman A, Dickson E, Rennie-Campbell L, Cross E, Heesom KJ, Mellor H
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2019 Nov;33(11):12277-12287

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Liprin-α1 modulates cancer cell signaling by transmembrane protein CD82 in adhesive membrane domains linked to cytoskeleton.

Pehkonen H, Lento M, von Nandelstadh P, Filippou A, Grénman R, Lehti K, Monni O
Cell communication and signaling : CCS 2018 Jul 13;16(1):41

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Identification of a membrane-less compartment regulating invadosome function and motility.

Sala K, Raimondi A, Tonoli D, Tacchetti C, de Curtis I
Scientific reports 2018 Jan 18;8(1):1164

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A Method to Culture GABAergic Interneurons Derived from the Medial Ganglionic Eminence.

Franchi SA, Macco R, Astro V, Tonoli D, Savino E, Valtorta F, Sala K, Botta M, de Curtis I
Frontiers in cellular neuroscience 2017;11:423

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Cell polarity defines three distinct domains in pancreatic β-cells.

Gan WJ, Zavortink M, Ludick C, Templin R, Webb R, Webb R, Ma W, Poronnik P, Parton RG, Gaisano HY, Shewan AM, Thorn P
Journal of cell science 2017 Jan 1;130(1):143-151

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Identification of a Protein Network Driving Neuritogenesis of MGE-Derived GABAergic Interneurons.

Franchi SA, Astro V, Macco R, Tonoli D, Barnier JV, Botta M, de Curtis I
Frontiers in cellular neuroscience 2016;10:289

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Liprin-α1 and ERC1 control cell edge dynamics by promoting focal adhesion turnover.

Astro V, Tonoli D, Chiaretti S, Badanai S, Sala K, Zerial M, de Curtis I
Scientific reports 2016 Sep 23;6:33653

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PPFIA1 drives active α5β1 integrin recycling and controls fibronectin fibrillogenesis and vascular morphogenesis.

Mana G, Clapero F, Panieri E, Panero V, Böttcher RT, Tseng HY, Saltarin F, Astanina E, Wolanska KI, Morgan MR, Humphries MJ, Santoro MM, Serini G, Valdembri D
Nature communications 2016 Nov 23;7:13546

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Effects of the scaffold proteins liprin-α1, β1 and β2 on invasion by breast cancer cells.

Chiaretti S, Astro V, Chiricozzi E, de Curtis I
Biology of the cell 2016 Mar;108(3):65-75

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Liprin-α1 is a regulator of vimentin intermediate filament network in the cancer cell adhesion machinery.

Pehkonen H, von Nandelstadh P, Karhemo PR, Lepikhova T, Grenman R, Lehti K, Monni O
Scientific reports 2016 Apr 14;6:24486

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Liprin-α1, ERC1 and LL5 define polarized and dynamic structures that are implicated in cell migration.

Astro V, Chiaretti S, Magistrati E, Fivaz M, de Curtis I
Journal of cell science 2014 Sep 1;127(Pt 17):3862-76

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Prognostic significance of TMEM16A, PPFIA1, and FADD expression in invasive ductal carcinoma of the breast.

Choi EJ, Yun JA, Jabeen S, Jeon EK, Won HS, Ko YH, Kim SY
World journal of surgical oncology 2014 May 1;12:137

Western blot

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

HeLa cells were subjected to SDS PAGE followed by western blot with 14175-1-AP(PPFIA1 antibody) at dilution of 1:500

Sample type

cell line

Immunocytochemistry

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunofluorescent analysis of HepG2 cells, using PPFIA1 antibody 14175-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

Sample type

cell line

Immunohistochemistry

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunohistochemical of paraffin-embedded human prostate cancer using 14175-1-AP(PPFIA1 antibody) at dilution of 1:100 (under 10x lens)

Sample type

tissue

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunohistochemical of paraffin-embedded human prostate cancer using 14175-1-AP(PPFIA1 antibody) at dilution of 1:100 (under 40x lens)

Sample type

tissue