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Learn14175-1-AP
antibody from Invitrogen Antibodies
Targeting: PPFIA1
LIP.1, LIPRIN
Western blot Immunocytochemistry
Immunoprecipitation Immunohistochemistry Flow cytometry Other assayAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
- Flow cytometry [1]
- Other assay [1]
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- Product number
- 14175-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Liprin alpha 1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: MMCEVMPTI SEAEGPPGGG GGHGSGSPSQ PDADSHFEQL MVSMLEERDR LLDTLRETQE TLALTQGKLH EVGHERDSLQ RQLNTALPQE FAALTKELNV CREQLLEREE EIAELKAERN NTRLLLEHLE CLVSRHERSL RMTVVKRQAQ SPAGVSSEVE VLKALKSLFE HHKALDEKVR ERLRVALERC SLLEEELGAT HKELMILKEQ NNQKKTLTDG VLDINHEQEN TPSTSGKRSS DGSLSHEEDL AKVIELQEII SKQSREQSQM KERLASLSSH VTELEEDLDT ARKDLIKSEE MNTKLQRDVR EAMAQKEDME ERITTLEKRY LAAQREATSV HDLNDKLENE IANKD (1-354 aa encoded by BC034046)
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.29 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- MCF7 cells were subjected to SDS PAGE followed by western blot with 14175-1-AP (Liprin alpha 1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HeLa cells were subjected to SDS PAGE followed by western blot with 14175-1-AP (Liprin alpha 1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of HepG2 cells, using PPFIA1 antibody 14175-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IGG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human prostate cancer using 14175-1-AP (Liprin alpha 1 antibody) at dilution of 1:100 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human prostate cancer using 14175-1-AP (Liprin alpha 1 antibody) at dilution of 1:100 (under 40x lens).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- 1X10^6 HeLa cells were stained with 0.2ug Liprin alpha 1 antibody (14175-1-AP, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L) with dilution 1:1500.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP result of anti-Liprin alpha 1 (IP:14175-1-AP, 4ug; Detection:14175-1-AP 1:500) with MCF-7 cells lysate 3200ug.
