PA5-22375

antibody from Invitrogen Antibodies

Targeting: PPFIA1 LIP.1, LIPRIN

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-22375

Provider

Invitrogen Antibodies

Product name

PPFIA1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant protein fragment

Description

Recommended positive controls: 293T, A431, HeLa, HepG2, JurKat, Neuro2A.

Concentration

0.98 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using PPFIA1 Polyclonal Antibody (Product # PA5-22375). Sample (30 µg of whole cell lysate). Lane A: JurKat . 5% SDS PAGE. PPFIA1 Polyclonal Antibody (Product # PA5-22375) diluted at 1:10,000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Liprin alpha 1 antibody detects Liprin alpha 1 protein by western blot analysis. Various whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with Liprin alpha 1 antibody PPFIA1 Polyclonal Antibody (Product # PA5-22375) diluted by 1:1,000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

PPFIA1 Polyclonal Antibody detects PPFIA1 protein by Western blot analysis. A. 30 µg Neuro2A whole cell lysate/extract.5 % SDS-PAGE. PPFIA1 Polyclonal Antibody (Product # PA5-22375) dilution: 1:1,000.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of PPFIA1 was performed in DIV9 rat E18 primary cortical neuron cells fixed in 4% paraformaldehyde at RT for 15 min. Green: PPFIA1 Polyclonal Antibody (Product # PA5-22375) diluted at 1:500. Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody. Blue: Fluoroshield with DAPI.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunoprecipitation of Liprin alpha 1 was performed in A431 whole cell extracts using 5 µg of PPFIA1 Polyclonal Antibody (Product # PA5-22375). Samples were transferred to a membrane and probed with PPFIA1 Polyclonal Antibody as a primary antibody and an HRP-conjugated anti-Rabbit IgG was used as a secondary antibody.