NB100-79784
antibody from Novus Biologicals
Targeting: BANP
BEND1, DKFZp761H172, FLJ10177, FLJ20538, SMAR1, SMARBP1
Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunoprecipitation [1]
- Immunohistochemistry [4]
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Validation data
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- Product number
- NB100-79784 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-79784, RRID:AB_1110519
- Product name
- Rabbit Polyclonal BANP Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ul
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C. Do not freeze.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: BANP Antibody [NB100-79784] - Detection of Human, Mouse and Rat SMAR1/BANP by Western Blot. Samples: Whole cell lysate (50 ug) from mouse 4T1, mouse CT26.WT, rat C6, HeLa, and 293T cells. Antibodies: Affinity purified rabbit anti-SMAR1/BANP antibody NB100-79784 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: BANP Antibody [NB100-79784] - Nuclear extract (NE) from HeLa cells. Antibody used at 0.1 ug/ml.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: BANP Antibody [NB100-79784] - Detection of human and mouse SMAR1 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-SMAR1 antibody NB100-79784 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: BANP Antibody [NB100-79784] - Detection of human SMAR1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-SMAR1 antibody NB100-79784 (lot NB100-79784-3) used for IP at 6 ug per reaction. SMAR1 was also immunoprecipitated by a previous lot of this antibody (lot NB100-79784-2). For blotting immunoprecipitated SMAR1, NB100-79784 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: BANP Antibody [NB100-79784] - Sample: FFPE sections of human lung carcinoma (left) and mouse renal cell carcinoma (right). Antibody: Affinity purified rabbit anti-fs18 SMAR1 fs20 used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: BANP Antibody [NB100-79784] - FFPE section of human breast tumor. Affinity purified rabbit anti-SMAR1/BANP used at a dilution of 1:250. Detection: DAB staining using Immunohistochemistry Accessory Kit
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: BANP Antibody [NB100-79784] - Detection of mouse SMAR1 by immunohistochemistry. Samples: FFPE sections of mouse renal cell carcinoma. Antibodies: Affinity purified rabbit anti-SMAR1 Cat. No. NB100-79784 Lot 2 used at a dilution of 1:5,000 (0.2ug/ml) (left) and Cat. No. NB100-79784 Lot3 used at a dilution of 1:5,000 (0.2ug/ml) (right). Detection: DAB
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: BANP Antibody [NB100-79784] - Detection of human SMAR1 by immunohistochemistry. Samples: FFPE sections of human lung carcinoma. Antibodies: Affinity purified rabbit anti-SMAR1 Cat. No. NB100-79784 Lot 2 used at a dilution of 1:5,000 (0.2ug/ml) (left) and Cat. No. NB100-79784 Lot3 used at a dilution of 1:5,000 (0.2ug/ml) (right). Detection: DAB