Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [3]
- ELISA [1]
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Validation data
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- Product number
- H00000558-M01 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00000558-M01, RRID:AB_537044
- Product name
- Mouse Monoclonal Axl Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. AXL - AXL receptor tyrosine kinase
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references Axl/Gas6/NFκB signalling in schwannoma pathological proliferation, adhesion and survival.
Gain-of-Function Activity of Mutant p53 in Lung Cancer through Up-Regulation of Receptor Protein Tyrosine Kinase Axl.
Ammoun S, Provenzano L, Zhou L, Barczyk M, Evans K, Hilton DA, Hafizi S, Hanemann CO
Oncogene 2014 Jan 16;33(3):336-46
Oncogene 2014 Jan 16;33(3):336-46
Gain-of-Function Activity of Mutant p53 in Lung Cancer through Up-Regulation of Receptor Protein Tyrosine Kinase Axl.
Vaughan CA, Singh S, Windle B, Yeudall WA, Frum R, Grossman SR, Deb SP, Deb S
Genes & cancer 2012 Jul;3(7-8):491-502
Genes & cancer 2012 Jul;3(7-8):491-502
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Axl Antibody (6C8) [H00000558-M01] - Analysis of AXL over-expressed 293 cell line, cotransfected with AXL Validated Chimera RNAi ( Cat # H00000558-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with AXL monoclonal antibody (M01) clone 6C8 (Cat # H00000558-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Axl Antibody (6C8) [H00000558-M01] - Analysis of AXL expression in transfected 293T cell line by AXL monoclonal antibody (M01), clone 6C8.Lane 1: AXL transfected lysate(98 KDa).Lane 2: Non-transfected lysate.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Axl Antibody (6C8) [H00000558-M01] - AXL monoclonal antibody (M01), clone 6C8 Analysis of AXL expression in HeLa.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- ELISA: Axl Antibody (6C8) [H00000558-M01] - Detection limit for recombinant GST tagged AXL is approximately 0.3ng/ml as a capture antibody.