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Western blot
ELISA
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Immunocytochemistry [2]
- Immunoprecipitation [1]
- Immunohistochemistry [1]
- Other assay [1]
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- Product number
- PA5-116782 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Axl Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Positive Samples: HeLa, SW620, HepG2 Immunogen sequence: EESPFVGNPG NITGARGLTG TLRCQLQVQG EPPEVHWLRD GQILELADST QTQVPLGEDE QDDWIVVSQL RITSLQLSDT GQYQCLVFLG HQTFVSQPGY VGLEGLPYFL EEPEDRTVAA NTPFNLSCQA QGPPEPVDLL WLQDAVPLAT APGHGPQRSL HVPGLNKTSS FSCEAHNAKG VTTSRTATIT VLPQQPRNLH LVSRQPTELE VAWTPGLSGI YPLTHCTLQA VLSDDGMGIQ AGEPDPPEEP LTSQASVPPH QLRLGSLHPH TPYHIRVACT SSQGPSSWTH WLPVETPEGV PLGPPENISA TRNGSQAFVH WQEPRAPLQG TLLGYRLAYQ GQDTPEVLMD IGLRQEVTLE LQGDGSVSNL TVCVAAYTAA GDGPWSLPVP LEAWRPGQAQ PVHQLVKEPS TPAFSWP
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 2.89 mg/mL
- Storage
- -20°C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of Axl in DU145 cells. Samples were incubated with Axl Polyclonal antibody (Product # PA5-116782) using a dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of Axl in HeLa cells. Samples were incubated with Axl Polyclonal antibody (Product # PA5-116782) using a dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation of Axl in 300 μg extracts of Hela cells. Samples were precipitated with 3 μg Axl Polyclonal antibody (Product # PA5-116782). Western blot was performed from the immunoprecipitate using Axl Polyclonal antibody (Product # PA5-116782) at a dilution of 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of Axl in paraffin-embedded human lung cancer. Samples were incubated with Axl Polyclonal antibody (Product # PA5-116782) using a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation analysis of Axl in 300 µg extracts of HeLa cells using Axl Polyclonal Antibody (Product # PA5-116782). Western blot was performed from the immunoprecipitate using the same antibody at a dilution of 1:100
