AGA-015-25UL

antibody from Invitrogen Antibodies

Targeting: GABRE

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

AGA-015-25UL

Provider

Invitrogen Antibodies

Product name

GABA(A) epsilon Receptor (GABRE) (extracellular) Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

25 µL

Concentration

0.85 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of rat brain membrane (lanes 1 and 5), mouse brain lysate (lanes 2 and 6), human SHSY-5Y neuroblastoma cells lysate (lanes 3 and 7) and human U87 MG glioblastoma cells lysate (lanes 4 and 8): - 1-4. Anti-GABA (A) epsilon Receptor (GABRE) (extracellular) Antibody (#AGA-015), (1:400).5-8. Anti-GABA (A) epsilon Receptor (GABRE) (extracellular) Antibody , preincubated with GABA (A) epsilon Receptor/GABRE (extracellular) Blocking Peptide (#BLP-GA015).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of rat brain membrane (lanes 1 and 5), mouse brain lysate (lanes 2 and 6), human SHSY-5Y neuroblastoma cells lysate (lanes 3 and 7) and human U87 MG glioblastoma cells lysate (lanes 4 and 8): - 1-4. Anti-GABA (A) epsilon Receptor (GABRE) (extracellular) Antibody (#AGA-015), (1:400).5-8. Anti-GABA (A) epsilon Receptor (GABRE) (extracellular) Antibody , preincubated with GABA (A) epsilon Receptor/GABRE (extracellular) Blocking Peptide (#BLP-GA015).

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Expression of GABA (A) epsilon receptor in human U-87 MG cells - Cell surface detection of GABA (A) epsilon receptor in intact living human U-87 MG cells. A. Extracellular staining of cells using Anti-GABA (A) epsilon Receptor (GABRE) (extracellular) Antibody (#AGA-015), (1:25) followed by goat Anti-rabbit-AlexaFluor-594 secondary Antibody , merged with nuclear staining using DAPI (blue). B. Extracellular staining (red) merged with live view of the cells.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Expression of GABA (A) epsilon receptor in rat and mouse cerebella - Immunohistochemical staining of mouse and rat cerebellum using Anti-GABA (A) epsilon Receptor (GABRE) (extracellular) Antibody (#AGA-015). In both mouse (A) and rat (B) cerebellum GABA (A) epsilon receptor staining (purple) appears in the Purkinje cell body (P and vertical arrows) and in their dendritic tree (horizontal arrow) extending into the molecular layer (MOL). Nuclear staining using DAPI as the counterstaining (blue).