Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Other assay [1]
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Validation data
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- Product number
- PA5-22164 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- MVD Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: 293T, A431, HeLa, A375, mouse Liver.
- Concentration
- 1 mg/mL
Submitted references ADAMTS1, MPDZ, MVD, and SEZ6: candidate genes for autosomal recessive nonsyndromic hearing impairment.
Bharadwaj T, Schrauwen I, Rehman S, Liaqat K, Acharya A, Giese APJ, Nouel-Saied LM, Nasir A, Everard JL, Pollock LM, Zhu S, Bamshad MJ, Nickerson DA, Ali RH, Ullah A, Wali A, Ali G, Santos-Cortez RLP, Ahmed ZM, McDermott BM Jr, Ansar M, Riazuddin S, Ahmad W, Leal SM
European journal of human genetics : EJHG 2022 Jan;30(1):22-33
European journal of human genetics : EJHG 2022 Jan;30(1):22-33
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using MVD Polyclonal Antibody (Product # PA5-22164). Sample (50 µg of whole cell lysate). Lane A: mouse Liver. 10% SDS PAGE. MVD Polyclonal Antibody (Product # PA5-22164) diluted at 1:1,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- MVD Polyclonal Antibody detects MVD protein by Western blot analysis. A. 30 µg 293T whole lysate/extract. B. 30 µg A431 whole cell lysate/extract. C. 30 µg HeLa whole cell lysate/extract. D. 30 µg A375 whole cell lysate/extract.10 % SDS-PAGE. MVD Polyclonal Antibody (Product # PA5-22164) dilution: 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of MVD in paraformaldehyde-fixed HeLa cells using a MVD polyclonal antibody (Product # PA5-22164) at a 1:200 dilution.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded MCF7 xenograft, using MVD (Product # PA5-22164) antibody at 1:100 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Fig. 4 Immunostaining of Adamts1, Mpdz, Mvd, and Sez6 in the inner ear of wildtype mice. A Whole mount immunostaining of Adamts1, Mpdz, and Sez6 in wildtype mice at P12. Immunoreactivity was visualized with a fluorescently labeled secondary antibody (red) and F-actin was stained with phalloidin 488 nm (green). Immunolabeling of Adamts1 and Sez6 is observed in stereocilia as well as cytoplasm of outer hair cells. Mpdz is observed in the cytoplasmic region of hair cells. B Immunostaining of the organ of Corti at the apical, medial and basal turns of the cochlea at P4. C Immunostaining of Mvd in wildtype mice at P1, P4, and P28. Immunoreactivity of Mvd was visualized with a fluorescently labeled secondary antibody (green), F-actin was stained with rhodamine-phalloidin (red) and nuclear bodies were stained with DAPI (blue). The anti-neurofilament (NF-200, purple) was used to mark the neurons. Immunolabeling of Mvd is observed in spiral ganglion cells (SG). HP Habenula perforata, OC Organ of Corti.