10758-1-AP

antibody from Proteintech Group

Targeting: HADHA GBP, LCEH, LCHAD, MTPA

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Data presented on provider website)

Antibody Data

Product number

10758-1-AP

Provider

Proteintech Group

Proper citation

Proteintech Cat#10758-1-AP, RRID:AB_2115593

Product name

HADHA antibody

Antibody type

Polyclonal

Description

KD/KO validated HADHA antibody (Cat. #10758-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: FC, IF, IHC, IP, WB, ELISA.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Conjugate

Unconjugated

Isotype

IgG

Vial size

20ul, 150ul

References

Submitted references

Integrated spatial multi-omics delineates fatty acid degradation fuels malignant evolution at the tumour periphery in cervical squamous cell carcinoma.

Lin S, Luo Z, Li Y, Hu B, Qu M, Liu B, Cao C, Peng T, Xu M, Xu Y, Liu X, Wang X, Li L, Ding W, Xu Z, Li R, Zhao J, Zhang J, Wang L, Ren F, Wang C, Wu P
EBioMedicine 2026 May;127:106256

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SIRT1 deficiency promotes age-related heart failure through enhancing ferroptosis via GATA4-HADHA-GPX4 axis.

Duan Y, Luo Y, Han X, Yu H, Liu H, Zhou Y, Gao Y, Xu Q, Wei Y, Min R, Hong Y, Ji X, Jia H, Li Y, Zhang Y
Cell death & disease 2026 Mar 23;17(1)

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Comprehensive multi-omics analysis revealed the mechanism of Panax ginseng extract in alleviating Qi deficiency liver cancer.

Wang M, Pei S, Wang R, Liu S, Hou Z, Liu Z
Journal of ethnopharmacology 2026 Mar 1;358:120985

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Mitochondrial fatty acid oxidation as the target for blocking therapy-resistance and inhibiting tumor recurrence: The proof-of-principle model demonstrated for ovarian cancer cells.

Lin H, Wang L, Chen H, Shen Y, Wang C, Xue Y, Zheng Z, Zhang Y, Xia D, Wu Y, Wang F, Li X, Cheng X, Wang H, Xu J, Lu W
Journal of advanced research 2026 Jan;79:571-585

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Mitochondrial protein-enriched artificial nanovesicles: mitochondrial recovery and antioxidation for diabetic wound treatment.

Xia J, Wang L, Song Y, Zhu M, Xu Y, Liu J, Guan X, Zhang Q, He K, Wang F, Liu L, Liu J
Journal of nanobiotechnology 2026 Feb 5;24(1)

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A galacturonan from sea buckthorn (Hippophae rhamnoides L.) berry modulates dyslipidemia by promoting hepatic lipolysis and mitochondrial oxidation.

Sun Y, Ni W, Wang D, Ren J, Zhao D, Yu S, Liu S, Zhang S, Wang S, Wang W, Liu M
Carbohydrate polymers 2025 Sep 1;363:123708

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Sex-dependent effects of a high-fat diet-induced obesity model on cerebrovascular health and brain metabolism.

Eminhizer NN, Lin D, Hanshew A, Stewart J, Ball S, Lilly C, Prabhu SS, Karelina K, Kelley EE, Bryner RW, Thapa D, Chantler PD
Experimental physiology 2025 Oct 3;

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Inhalation of Ultrafine Carbon Black-Induced Mitochondrial Dysfunction in Mouse Heart Through Changes in Acetylation.

Islam R, Stewart JE, Mullen WE, Lin D, Hussain S, Thapa D
Cells 2025 Nov 4;14(21)

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Multiorgan proteomic analysis of infected animal models predict potential host factors for chikungunya virus.

Lin D, Tang C, Wang J, Yang Y, Yang H, Zhou Y, Yu W, Li B, Huang Q, Wang H, An R, Liang X, Yan Y, Yuan L, Du X, Yuan Y, Li Y, Lu S
MedComm 2025 Jan;6(1):e70013

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Moderate-intensity interval exercise exacerbates cardiac lipotoxicity in high-fat, high-calories diet-fed mice.

Geng J, Zhang X, Guo Y, Wen H, Guo D, Liang Q, Pu S, Wang Y, Liu M, Li Z, Hu W, Yang X, Chang P, Hu L, Li Y
Nature communications 2025 Jan 12;16(1):613

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Putative Role of Fatty Acid Metabolic Therapy Using Ketogenic Diet and HIF-1α Inhibition in Hepatocellular Carcinoma: Evidence from an In Vitro Study.

Kimura N, Kitajima Y, Higure K, Matsufuji S, Ikeda S, Furukawa S, Yukimoto K, Tanaka T, Noshiro H
International journal of molecular sciences 2025 Dec 5;26(24)

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Cardiomyocyte-specific deletion of GCN5L1 reduces lysine acetylation and attenuates diastolic dysfunction in aged mice by improving cardiac fatty acid oxidation.

Stewart JE, Crawford JM, Mullen WE, Jacques A, Stoner MW, Scott I, Thapa D
The Biochemical journal 2024 Mar 20;481(6):423-436

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Tamoxifen upregulates the peroxisomal β-oxidation enzyme Enoyl CoA hydratase and 3-hydroxyacyl CoA hydratase ameliorating hepatic lipid accumulation in mice.

Zhang Z, Yang Q, Jin M, Wang J, Chai Y, Zhang L, Jiang Z, Yu Q
The international journal of biochemistry & cell biology 2024 Jul;172:106585

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Protein lysine acetylation played an important role in NH(3)-induced AEC2 damage and pulmonary fibrosis in piglets.

Wang X, Zhang D, Zhu Y, Li D, Shen L, Wang Q, Gao Y, Li X, Yu M
The Science of the total environment 2024 Jan 15;908:168303

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Regulation of Na+-K+-ATPase leads to disturbances of isoproterenol-induced cardiac dysfunction via interference of Ca2+-dependent cardiac metabolism.

Yan X, Li M, Lan P, Xun M, Zhang Y, Shi J, Wang R, Zheng J
Clinical science (London, England : 1979) 2024 Jan 10;138(1):23-42

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Comparison of the stage-dependent mitochondrial changes in response to pressure overload between the diseased right and left ventricle in the rat.

Li L, Niemann B, Knapp F, Werner S, Mühlfeld C, Schneider JP, Jurida LM, Molenda N, Schmitz ML, Yin X, Mayr M, Schulz R, Kracht M, Rohrbach S
Basic research in cardiology 2024 Aug;119(4):587-611

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miR-612 Enhances RSL3-Induced Ferroptosis of Hepatocellular Carcinoma Cells via Mevalonate Pathway.

Xing K, Bian X, Shi D, Dong S, Zhou H, Xiao S, Bai J, Wu W
Journal of hepatocellular carcinoma 2023;10:2173-2185

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F-box only protein 2 exacerbates non-alcoholic fatty liver disease by targeting the hydroxyl CoA dehydrogenase alpha subunit.

Liu Z, Chen NY, Zhang Z, Zhou S, Hu SY
World journal of gastroenterology 2023 Jul 28;29(28):4433-4450

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G-protein coupled receptor 19 (GPR19) knockout mice display sex-dependent metabolic dysfunction.

Mushala BAS, Xie B, Sipula IJ, Stoner MW, Thapa D, Manning JR, Bugga P, Vandevender AM, Jurczak MJ, Scott I
Scientific reports 2023 Apr 15;13(1):6134

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Dynamic Mitochondrial Proteome Under Polyamines Treatment in Cardiac Aging.

Zhang H, Yan M, Liu T, Wei P, Chai N, Li L, Wang J, Yu X, Lin Y, Qiu B, Zhao Y
Frontiers in cell and developmental biology 2022;10:840389

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3,4-Dihydroxyphenylethanol (DPE or Hydroxytyrosol) Counteracts ERK1/2 and mTOR Activation, Pro-Inflammatory Cytokine Release, Autophagy and Mitophagy Reduction Mediated by Benzo[a]pyrene in Primary Human Colonic Epithelial Cells.

Santarelli R, Pompili C, Gilardini Montani MS, Evangelista L, Gonnella R, Cirone M
Pharmaceutics 2022 Mar 17;14(3)

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Cardioprotective Role of SIRT5 in Response to Acute Ischemia Through a Novel Liver-Cardiac Crosstalk Mechanism.

Zhou B, Xiao M, Hu H, Pei X, Xue Y, Miao G, Wang J, Li W, Du Y, Zhang P, Wei T
Frontiers in cell and developmental biology 2021;9:687559

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The Impaired Bioenergetics of Diabetic Cardiac Microvascular Endothelial Cells.

Zhang H, Shen Y, Kim IM, Weintraub NL, Tang Y
Frontiers in endocrinology 2021;12:642857

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Feedback repression of PPARα signaling by Let-7 microRNA.

Yagai T, Yan T, Luo Y, Takahashi S, Aibara D, Kim D, Brocker CN, Levi M, Motohashi H, Gonzalez FJ
Cell reports 2021 Aug 10;36(6):109506

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Mild Hypothermia Attenuates Hepatic Ischemia-Reperfusion Injury through Regulating the JAK2/STAT3-CPT1a-Dependent Fatty Acid β-Oxidation.

Wang W, Hu X, Xia Z, Liu Z, Zhong Z, Lu Z, Liu A, Ye S, Cao Q, Wang Y, Zhu F, Ye Q
Oxidative medicine and cellular longevity 2020;2020:5849794

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Rescue of myocardial energetic dysfunction in diabetes through the correction of mitochondrial hyperacetylation by honokiol.

Kerr M, Miller JJ, Thapa D, Stiewe S, Timm KN, Aparicio CNM, Scott I, Tyler DJ, Heather LC
JCI insight 2020 Sep 3;5(17)

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High-Dose Dexamethasone Manipulates the Tumor Microenvironment and Internal Metabolic Pathways in Anti-Tumor Progression.

Xu L, Xia H, Ni D, Hu Y, Liu J, Qin Y, Zhou Q, Yi Q, Xie Y
International journal of molecular sciences 2020 Mar 7;21(5)

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The protein acetylase GCN5L1 modulates hepatic fatty acid oxidation activity via acetylation of the mitochondrial β-oxidation enzyme HADHA.

Thapa D, Wu K, Stoner MW, Xie B, Zhang M, Manning JR, Lu Z, Li JH, Chen Y, Gucek M, Playford MP, Mehta NN, Harmon D, O'Doherty RM, Jurczak MJ, Sack MN, Scott I
The Journal of biological chemistry 2018 Nov 16;293(46):17676-17684

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Establishment of a novel hepatic steatosis cell model by Cas9/sgRNA-mediated DGKθ gene knockout.

Zhang J, Zhao J, Zheng X, Cai K, Mao Q, Xia H
Molecular medicine reports 2018 Feb;17(2):2169-2176

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Succinate accumulation impairs cardiac pyruvate dehydrogenase activity through GRP91-dependent and independent signaling pathways: Therapeutic effects of ginsenoside Rb1.

Li J, Yang YL, Li LZ, Zhang L, Liu Q, Liu K, Li P, Liu B, Qi LW
Biochimica et biophysica acta. Molecular basis of disease 2017 Nov;1863(11):2835-2847

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Acetylation of mitochondrial proteins by GCN5L1 promotes enhanced fatty acid oxidation in the heart.

Thapa D, Zhang M, Manning JR, Guimarães DA, Stoner MW, O'Doherty RM, Shiva S, Scott I
American journal of physiology. Heart and circulatory physiology 2017 Aug 1;313(2):H265-H274

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Mitochondrial Sirtuin Network Reveals Dynamic SIRT3-Dependent Deacetylation in Response to Membrane Depolarization.

Yang W, Nagasawa K, Münch C, Xu Y, Satterstrom K, Jeong S, Hayes SD, Jedrychowski MP, Vyas FS, Zaganjor E, Guarani V, Ringel AE, Gygi SP, Harper JW, Haigis MC
Cell 2016 Nov 3;167(4):985-1000.e21

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Effect of high-fat diet on hepatic proteomics of hamsters.

Liao CC, Lin YL, Kuo CF
Journal of agricultural and food chemistry 2015 Feb 18;63(6):1869-81

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DNAJC19, a mitochondrial cochaperone associated with cardiomyopathy, forms a complex with prohibitins to regulate cardiolipin remodeling.

Richter-Dennerlein R, Korwitz A, Haag M, Tatsuta T, Dargazanli S, Baker M, Decker T, Lamkemeyer T, Rugarli EI, Langer T
Cell metabolism 2014 Jul 1;20(1):158-71

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The trifunctional protein mediates thyroid hormone receptor-dependent stimulation of mitochondria metabolism.

Chocron ES, Sayre NL, Holstein D, Saelim N, Ibdah JA, Dong LQ, Zhu X, Cheng SY, Lechleiter JD
Molecular endocrinology (Baltimore, Md.) 2012 Jul;26(7):1117-28

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Pressure load: the main factor for altered gene expression in right ventricular hypertrophy in chronic hypoxic rats.

Baandrup JD, Markvardsen LH, Peters CD, Schou UK, Jensen JL, Magnusson NE, Ørntoft TF, Kruhøffer M, Simonsen U
PloS one 2011 Jan 5;6(1):e15859

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Men supplemented with 17beta-estradiol have increased beta-oxidation capacity in skeletal muscle.

Maher AC, Akhtar M, Tarnopolsky MA
Physiological genomics 2010 Aug;42(3):342-7

---

Women have higher protein content of beta-oxidation enzymes in skeletal muscle than men.

Maher AC, Akhtar M, Vockley J, Tarnopolsky MA
PloS one 2010 Aug 6;5(8):e12025

Western blot

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

mouse kidney tissue were subjected to SDS PAGE followed by western blot with 10758-1-AP(HADHA antibody) at dilution of 1:1000

Sample type

tissue

Immunocytochemistry

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunofluorescent analysis of HepG2 cells, using HADHA antibody 10758-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

Sample type

cell line

Immunoprecipitation

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

IP Result of anti-HADHA (IP:10758-1-AP, 4ug; Detection:10758-1-AP 1:200) with HEK-293 cells lysate 2500ug.

Sample type

cell line

Immunohistochemistry

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunohistochemical of paraffin-embedded human colon cancer using 10758-1-AP(HADHA antibody) at dilution of 1:100 (under 40x lens)

Sample type

tissue