PA5-27348

antibody from Invitrogen Antibodies

Targeting: HADHA GBP, LCEH, LCHAD, MTPA

Western blot (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-27348

Provider

Invitrogen Antibodies

Product name

HADHA Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant protein fragment

Description

Recommended positive controls: Molt-4, Raji, mouse kidney, rat liver. Predicted reactivity: Mouse (86%), Rat (84%), Pig (86%), Sheep (88%), Rhesus Monkey (99%), Bovine (87%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

PKA Activates AMPK Through LKB1 Signaling in Follicular Thyroid Cancer.

Kari S, Vasko VV, Priya S, Kirschner LS
Frontiers in endocrinology 2019;10:769

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of HADHA was performed in HeLa cells fixed in ice-cold MeOH for 5 min. Green: HADHA Polyclonal Antibody (Product # PA5-27348) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 µm.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 2 Mouse tumors shown activation of mTOR and AMPK pathways. Representative mouse tumors from the genotypes shown at the top were analyzed by immunohistochemistry for the proteins shown at left. WT, Pten-TpoKO, and R1a-TpoKO mice were 1 year of age, whereas DRP-TpoKO mice were 6 months of age. All photos were taken at the same magnification, with scale bars shown in two panels in the left column.