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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- AP17454PU-N - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#AP17454PU-N, RRID:AB_10658713
- Product name
- anti HADHA (C-term)
- Antibody type
- Polyclonal
- Antigen
- KLH conjugated synthetic peptide between 734~763 amino acids from the C-terminal region of human HADHA
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Vial size
- 0.4 ml
- Concentration
- lot specific
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Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Western blot analysis of HADHA Antibody (C-term) Cat.-No AP17454PU-NÂ in A2058, A375, NCI-H460, Ramos cell line lysates (35ug/lane). HADHA (arrow) was detected using the purified Pab
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human lung carcinoma reacted with HADHA Antibody (C-term) Cat.-No AP17454PU-N, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Flow cytometry analysis of Ramos cells using HADHA Antibody (C-term) Cat.-No AP17454PU-N (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
