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Western blot
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- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [2]
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- Product number
- AF5106 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Goat Polyclonal PLA2G7/PAF-AH/Lp-PLA2 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects human PLA2G7/PAF-AH/Lp-PLA2 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) PLA2G-2A, recombinant mouse PLA2G-2A, and rhPLA2G-1B is observed.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references Differential expression of Lp-PLA2 in obesity and type 2 diabetes and the influence of lipids.
Jackisch L, Kumsaiyai W, Moore JD, Al-Daghri N, Kyrou I, Barber TM, Randeva H, Kumar S, Tripathi G, McTernan PG
Diabetologia 2018 May;61(5):1155-1166
Diabetologia 2018 May;61(5):1155-1166
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Human PLA2G7/PAF-AH/Lp-PLA2 by Western Blot. Western blot shows lysates of human plasma. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human PLA2G7/PAF-AH/Lp-PLA2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5106) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for PLA2G7/PAF-AH/Lp-PLA2 at approximately 29 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Human PLA2G7/PAF-AH/Lp-PLA2 by Simple WesternTM. Simple Western lane view shows human plasma, loaded at 1:10 dilution. A specific band was detected for PLA2G7/PAF-AH/Lp-PLA2 at approximately 33 kDa (as indicated) using 10 µg/mL of Goat Anti-Human PLA2G7/PAF-AH/Lp-PLA2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5106) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
