NBP2-14998

antibody from Novus Biologicals

Targeting: ISCU hnifU, ISU2, NIFUN

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

NBP2-14998

Provider

Novus Biologicals

Product name

Rabbit Polyclonal ISCU Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified.

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

0.1 ml

Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: ISCU Antibody [NBP2-14998] - Sample (30 ug of whole cell lysate) A: HeLa 12% SDS PAGE; antibody diluted at 1:1000.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: ISCU Antibody [NBP2-14998] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with ISCU antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: ISCU Antibody [NBP2-14998] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with ISCU antibody [N1C3] diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: ISCU Antibody [NBP2-14998] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with ISCU antibody [N1C3] diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: ISCU Antibody [NBP2-14998] - Paraffin-embedded A549 xenograft, using antibody at 1:500 dilution.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: ISCU Antibody [NBP2-14998] - Paraffin-embedded mouse muscle. ISCU antibody [N1C3] dilution: 1:500.