14812-1-AP

antibody from Proteintech Group

Targeting: ISCU hnifU, ISU2, NIFUN

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

14812-1-AP

Provider

Proteintech Group

Proper citation

Proteintech Cat#14812-1-AP, RRID:AB_2280362

Product name

ISCU antibody

Antibody type

Polyclonal

Description

KD/KO validated ISCU antibody (Cat. #14812-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IF, IHC, IP, WB,ELISA.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Conjugate

Unconjugated

Isotype

IgG

Vial size

20ul, 150ul

References

Submitted references

Sulfur partitioning from cysteine controls T cell proliferation and effector function.

Kelly B, Cha M, Gremelspacher T, Martin JL, Andreis M, Maloo I, Carrizo GE, Gidley M, Stanczak MA, Apostolova P, Longo J, DeCamp LM, Ma EH, Sheldon RD, Jones RG, Sanin DE, Majumdar A, Pearce EL
Cell 2026 Mar 31;

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Distinct control of T cell proliferation and effector function by partitioning of intracellular sulfur from cysteine.

Kelly B, Cha M, Gremelspacher T, Martin JL, Andreis M, Carrizo GE, Gidley M, Stanczak MA, Apostolova P, Sanin DE, Majumdar A, Pearce EL
bioRxiv : the preprint server for biology 2026 Jan 29;

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miR-210-3p Ameliorates Metabolic Dysfunction-Associated Steatohepatitis-Related Fibrosis by Targeting ISCU and Induces Ferroptosis.

Dai Z, Sun Z, Chen Q, Li Y, Sun Y, Jiao M, Yin X, Liu R, Liang H, Zhu C
Liver international : official journal of the International Association for the Study of the Liver 2025 Sep;45(9):e70282

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Iron Replacement Attenuates Hypoxic Pulmonary Hypertension by Remodeling Energy Metabolism via Regulating the HIF2α/Mitochondrial Complex I, III/ROS Axis.

Geng Y, Wang H, Bai Z, Ge R
Biomolecules 2025 May 21;15(5)

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Causal role of mitochondrial proteins in aortic aneurysms: Evidence from Mendelian randomization, transcriptomic analysis, and experimental validation.

Abodulikemu A, Li L, Juaiti M
Medicine 2025 Mar 7;104(10):e41757

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Antioxidant capacity of the iron-sulfur cluster assembly protein IscU2 is mediated by aspartate metabolism to promote tumor survival.

Yang X, Liang N, Liu D, Yan J, Yang X, Lv J, Xiao S, Wei X, Chen X, Yang Z, Gui S, Jin L, Yu S, Lyu J, Ren X
The Journal of biological chemistry 2025 Jun;301(6):110234

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ISCU-p53 axis orchestrates macrophage polarization to dictate immunotherapy response in esophageal squamous cell carcinoma.

Luo J, Zhang X, Liang Z, Zhuang W, Jiang M, Ma M, Peng S, Huang S, Qiao G, Chen Q
Cell death & disease 2025 Jun 20;16(1):462

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Identification and validation of mitochondrial ferroptosis and immune microenvironment-related hub biomarkers in liver cirrhosis by integrated bioinformatics analysis.

Deng P, Chaulagain RP, Oluwaseun BD, Gao F, Wang J, Gao R, Jiang X, Li F, Xu L, Xu H, Yao K, Jin S
Science progress 2025 Jul-Sep;108(3):368504251380638

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Impaired Iron-Sulfur Cluster Synthesis Induces Mitochondrial PARthanatos in Diabetic Cardiomyopathy.

Wang M, Zhang S, Tian J, Yang F, Chen H, Bai S, Kang J, Pang K, Huang J, Dong M, Dong S, Tian Z, Fang S, Fan H, Lu F, Yu B, Li S, Zhang W
Advanced science (Weinheim, Baden-Wurttemberg, Germany) 2025 Jan;12(1):e2406695

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Downregulation of the Zinc Transporter ZIP13 (Slc39a13) Leads to Ferroptosis by Inhibiting Mitochondrial Iron-Sulfur Cluster Biosynthesis and Induces Ischemia/Reperfusion Injury in Mouse Hearts.

Zhang R, Wang J, Yang Q, Yu Y, Cheng X, Xu Z
Antioxidants & redox signaling 2025 Aug;43(4-6):328-344

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MicroRNA210 Suppresses Mitochondrial Metabolism and Promotes Microglial Activation in Neonatal Hypoxic-Ischemic Brain Injury.

Hu S, Lopez-Robles Y, Shen G, Liu E, Zhang L, Ma Q
Cells 2025 Aug 5;14(15)

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Construction and multiple validations of a robust ferroptosis-related prognostic model in bladder cancer: A comprehensive study.

Dai X, Yu K, Wang H, Zhong R, Zhang Z, Hou Y
Medicine 2024 Oct 18;103(42):e40133

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Mitoferrin2 is a synthetic lethal target for chromosome 8p deleted cancers.

Krieg S, Rohde T, Rausch T, Butthof L, Wendler-Link L, Eckert C, Breuhahn K, Galy B, Korbel J, Billmann M, Breinig M, Tschaharganeh DF
Genome medicine 2024 Jun 17;16(1):83

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A mitochondrial iron-responsive pathway regulated by DELE1.

Sekine Y, Houston R, Eckl EM, Fessler E, Narendra DP, Jae LT, Sekine S
Molecular cell 2023 Jun 15;83(12):2059-2076.e6

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Ferrodifferentiation regulates neurodevelopment via ROS generation.

Chang S, Wang P, Han Y, Ma Q, Liu Z, Zhong S, Lu Y, Chen R, Sun L, Wu Q, Gao G, Wang X, Chang YZ
Science China. Life sciences 2023 Aug;66(8):1841-1857

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Salidroside inhibits doxorubicin-induced cardiomyopathy by modulating a ferroptosis-dependent pathway.

Chen H, Zhu J, Le Y, Pan J, Liu Y, Liu Z, Wang C, Dou X, Lu D
Phytomedicine : international journal of phytotherapy and phytopharmacology 2022 May;99:153964

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Frataxin controls ketone body metabolism through regulation of OXCT1.

Dong YN, Mesaros C, Xu P, Mercado-Ayón E, Halawani S, Ngaba LV, Warren N, Sleiman P, Rodden LN, Schadt KA, Blair IA, Lynch DR
PNAS nexus 2022 Jul;1(3):pgac142

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Skeletal muscle MiR-210 expression is associated with mitochondrial function in peripheral artery disease patients.

Ismaeel A, Fletcher E, Miserlis D, Wechsler M, Papoutsi E, Haynatzki G, Smith RS, Bohannon WT, Koutakis P
Translational research : the journal of laboratory and clinical medicine 2022 Aug;246:66-77

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Quercetin Antagonizes Glucose Fluctuation Induced Renal Injury by Inhibiting Aerobic Glycolysis via HIF-1α/miR-210/ISCU/FeS Pathway.

Xu WL, Liu S, Li N, Ye LF, Zha M, Li CY, Zhao Y, Pu Q, Bao JJ, Chen XJ, Yu JY, Pei YH
Frontiers in medicine 2021;8:656086

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Macrophage miR-210 induction and metabolic reprogramming in response to pathogen interaction boost life-threatening inflammation.

Virga F, Cappellesso F, Stijlemans B, Henze AT, Trotta R, Van Audenaerde J, Mirchandani AS, Sanchez-Garcia MA, Vandewalle J, Orso F, Riera-Domingo C, Griffa A, Ivan C, Smits E, Laoui D, Martelli F, Langouche L, Van den Berghe G, Feron O, Ghesquière B, Prenen H, Libert C, Walmsley SR, Corbet C, Van Ginderachter JA, Ivan M, Taverna D, Mazzone M
Science advances 2021 May;7(19)

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Rats with a Human Mutation of NFU1 Develop Pulmonary Hypertension.

Niihori M, Eccles CA, Kurdyukov S, Zemskova M, Varghese MV, Stepanova AA, Galkin A, Rafikov R, Rafikova O
American journal of respiratory cell and molecular biology 2020 Feb;62(2):231-242

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NDUFAB1 confers cardio-protection by enhancing mitochondrial bioenergetics through coordination of respiratory complex and supercomplex assembly.

Hou T, Zhang R, Jian C, Ding W, Wang Y, Ling S, Ma Q, Hu X, Cheng H, Wang X
Cell research 2019 Sep;29(9):754-766

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GRP75 overexpression rescues frataxin deficiency and mitochondrial phenotypes in Friedreich ataxia cellular models.

Dong YN, McMillan E, Clark EM, Lin H, Lynch DR
Human molecular genetics 2019 May 15;28(10):1594-1607

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Phosphoinositide-3-kinase inhibition elevates ferritin level resulting depletion of labile iron pool and blocking of glioma cell proliferation.

Gupta P, Singh P, Pandey HS, Seth P, Mukhopadhyay CK
Biochimica et biophysica acta. General subjects 2019 Mar;1863(3):547-564

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NDUFAB1 protects against obesity and insulin resistance by enhancing mitochondrial metabolism.

Zhang R, Hou T, Cheng H, Wang X
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2019 Dec;33(12):13310-13322

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MicroRNA-210 Downregulates ISCU and Induces Mitochondrial Dysfunction and Neuronal Death in Neonatal Hypoxic-Ischemic Brain Injury.

Ma Q, Dasgupta C, Li Y, Huang L, Zhang L
Molecular neurobiology 2019 Aug;56(8):5608-5625

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The Favored Mechanism for Coping with Acute Cold Stress: Upregulation of miR-210 in Rats.

Guo W, Lian S, Zhen L, Zang S, Chen Y, Lang L, Xu B, Guo J, Ji H, Wang J, Fu S, Zhang L, Yang H
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology 2018;46(5):2090-2102

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Dysfunction in the mitochondrial Fe-S assembly machinery leads to formation of the chemoresistant truncated VDAC1 isoform without HIF-1α activation.

Ferecatu I, Canal F, Fabbri L, Mazure NM, Bouton C, Golinelli-Cohen MP
PloS one 2018;13(3):e0194782

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Iron accumulation in senescent cells is coupled with impaired ferritinophagy and inhibition of ferroptosis.

Masaldan S, Clatworthy SAS, Gamell C, Meggyesy PM, Rigopoulos AT, Haupt S, Haupt Y, Denoyer D, Adlard PA, Bush AI, Cater MA
Redox biology 2018 Apr;14:100-115

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Targeted Metabolomics Reveals Abnormal Hepatic Energy Metabolism by Depletion of β-Carotene Oxygenase 2 in Mice.

Wu L, Guo X, Lyu Y, Clarke SL, Lucas EA, Smith BJ, Hildebrand D, Wang W, Medeiros DM, Shen X, Lin D
Scientific reports 2017 Nov 7;7(1):14624

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Regulation of iron homeostasis by the p53-ISCU pathway.

Funauchi Y, Tanikawa C, Yi Lo PH, Mori J, Daigo Y, Takano A, Miyagi Y, Okawa A, Nakamura Y, Matsuda K
Scientific reports 2015 Nov 12;5:16497

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The diabetes drug target MitoNEET governs a novel trafficking pathway to rebuild an Fe-S cluster into cytosolic aconitase/iron regulatory protein 1.

Ferecatu I, Gonçalves S, Golinelli-Cohen MP, Clémancey M, Martelli A, Riquier S, Guittet E, Latour JM, Puccio H, Drapier JC, Lescop E, Bouton C
The Journal of biological chemistry 2014 Oct 10;289(41):28070-86

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Cochaperone binding to LYR motifs confers specificity of iron sulfur cluster delivery.

Maio N, Singh A, Uhrigshardt H, Saxena N, Tong WH, Rouault TA
Cell metabolism 2014 Mar 4;19(3):445-57

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MicroRNA-210 decreases heme levels by targeting ferrochelatase in cardiomyocytes.

Qiao A, Khechaduri A, Kannan Mutharasan R, Wu R, Nagpal V, Ardehali H
Journal of the American Heart Association 2013 Apr 22;2(2):e000121

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MMS19 assembles iron-sulfur proteins required for DNA metabolism and genomic integrity.

Stehling O, Vashisht AA, Mascarenhas J, Jonsson ZO, Sharma T, Netz DJ, Pierik AJ, Wohlschlegel JA, Lill R
Science (New York, N.Y.) 2012 Jul 13;337(6091):195-9

Western blot

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

mouse heart tissue were subjected to SDS PAGE followed by western blot with 14812-1-AP(ISCU antibody) at dilution of 1:500

Sample type

tissue

Immunocytochemistry

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunofluorescent analysis of MCF-7 cells, using ISCU antibody 14812-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

Sample type

cell line

Immunoprecipitation

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

IP Result of anti-ISCU (IP:14812-1-AP, 3ug; Detection:14812-1-AP 1:500) with mouse heart tissue lysate 4000ug.

Sample type

tissue

Immunohistochemistry

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunohistochemical of paraffin-embedded human lung cancer using 14812-1-AP(ISCU antibody) at dilution of 1:50 (under 40x lens)

Sample type

tissue