Explore
Validate
Learn
Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [17]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
- Immunohistochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- 14812-1-AP - Provider product page
- Provider
- Proteintech Group
- Proper citation
- Proteintech Cat#14812-1-AP, RRID:AB_2280362
- Product name
- ISCU antibody
- Antibody type
- Polyclonal
- Description
- KD/KO validated ISCU antibody (Cat. #14812-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IF, IHC, IP, WB,ELISA.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 20ul, 150ul
Submitted references Quercetin Antagonizes Glucose Fluctuation Induced Renal Injury by Inhibiting Aerobic Glycolysis via HIF-1α/miR-210/ISCU/FeS Pathway.
Macrophage miR-210 induction and metabolic reprogramming in response to pathogen interaction boost life-threatening inflammation.
Rats with a Human Mutation of NFU1 Develop Pulmonary Hypertension.
NDUFAB1 confers cardio-protection by enhancing mitochondrial bioenergetics through coordination of respiratory complex and supercomplex assembly.
GRP75 overexpression rescues frataxin deficiency and mitochondrial phenotypes in Friedreich ataxia cellular models.
Phosphoinositide-3-kinase inhibition elevates ferritin level resulting depletion of labile iron pool and blocking of glioma cell proliferation.
NDUFAB1 protects against obesity and insulin resistance by enhancing mitochondrial metabolism.
MicroRNA-210 Downregulates ISCU and Induces Mitochondrial Dysfunction and Neuronal Death in Neonatal Hypoxic-Ischemic Brain Injury.
The Favored Mechanism for Coping with Acute Cold Stress: Upregulation of miR-210 in Rats.
Dysfunction in the mitochondrial Fe-S assembly machinery leads to formation of the chemoresistant truncated VDAC1 isoform without HIF-1α activation.
Iron accumulation in senescent cells is coupled with impaired ferritinophagy and inhibition of ferroptosis.
Targeted Metabolomics Reveals Abnormal Hepatic Energy Metabolism by Depletion of β-Carotene Oxygenase 2 in Mice.
Regulation of iron homeostasis by the p53-ISCU pathway.
The diabetes drug target MitoNEET governs a novel trafficking pathway to rebuild an Fe-S cluster into cytosolic aconitase/iron regulatory protein 1.
Cochaperone binding to LYR motifs confers specificity of iron sulfur cluster delivery.
MicroRNA-210 decreases heme levels by targeting ferrochelatase in cardiomyocytes.
MMS19 assembles iron-sulfur proteins required for DNA metabolism and genomic integrity.
Xu WL, Liu S, Li N, Ye LF, Zha M, Li CY, Zhao Y, Pu Q, Bao JJ, Chen XJ, Yu JY, Pei YH
Frontiers in medicine 2021;8:656086
Frontiers in medicine 2021;8:656086
Macrophage miR-210 induction and metabolic reprogramming in response to pathogen interaction boost life-threatening inflammation.
Virga F, Cappellesso F, Stijlemans B, Henze AT, Trotta R, Van Audenaerde J, Mirchandani AS, Sanchez-Garcia MA, Vandewalle J, Orso F, Riera-Domingo C, Griffa A, Ivan C, Smits E, Laoui D, Martelli F, Langouche L, Van den Berghe G, Feron O, Ghesquière B, Prenen H, Libert C, Walmsley SR, Corbet C, Van Ginderachter JA, Ivan M, Taverna D, Mazzone M
Science advances 2021 May;7(19)
Science advances 2021 May;7(19)
Rats with a Human Mutation of NFU1 Develop Pulmonary Hypertension.
Niihori M, Eccles CA, Kurdyukov S, Zemskova M, Varghese MV, Stepanova AA, Galkin A, Rafikov R, Rafikova O
American journal of respiratory cell and molecular biology 2020 Feb;62(2):231-242
American journal of respiratory cell and molecular biology 2020 Feb;62(2):231-242
NDUFAB1 confers cardio-protection by enhancing mitochondrial bioenergetics through coordination of respiratory complex and supercomplex assembly.
Hou T, Zhang R, Jian C, Ding W, Wang Y, Ling S, Ma Q, Hu X, Cheng H, Wang X
Cell research 2019 Sep;29(9):754-766
Cell research 2019 Sep;29(9):754-766
GRP75 overexpression rescues frataxin deficiency and mitochondrial phenotypes in Friedreich ataxia cellular models.
Dong YN, McMillan E, Clark EM, Lin H, Lynch DR
Human molecular genetics 2019 May 15;28(10):1594-1607
Human molecular genetics 2019 May 15;28(10):1594-1607
Phosphoinositide-3-kinase inhibition elevates ferritin level resulting depletion of labile iron pool and blocking of glioma cell proliferation.
Gupta P, Singh P, Pandey HS, Seth P, Mukhopadhyay CK
Biochimica et biophysica acta. General subjects 2019 Mar;1863(3):547-564
Biochimica et biophysica acta. General subjects 2019 Mar;1863(3):547-564
NDUFAB1 protects against obesity and insulin resistance by enhancing mitochondrial metabolism.
Zhang R, Hou T, Cheng H, Wang X
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2019 Dec;33(12):13310-13322
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2019 Dec;33(12):13310-13322
MicroRNA-210 Downregulates ISCU and Induces Mitochondrial Dysfunction and Neuronal Death in Neonatal Hypoxic-Ischemic Brain Injury.
Ma Q, Dasgupta C, Li Y, Huang L, Zhang L
Molecular neurobiology 2019 Aug;56(8):5608-5625
Molecular neurobiology 2019 Aug;56(8):5608-5625
The Favored Mechanism for Coping with Acute Cold Stress: Upregulation of miR-210 in Rats.
Guo W, Lian S, Zhen L, Zang S, Chen Y, Lang L, Xu B, Guo J, Ji H, Wang J, Fu S, Zhang L, Yang H
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology 2018;46(5):2090-2102
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology 2018;46(5):2090-2102
Dysfunction in the mitochondrial Fe-S assembly machinery leads to formation of the chemoresistant truncated VDAC1 isoform without HIF-1α activation.
Ferecatu I, Canal F, Fabbri L, Mazure NM, Bouton C, Golinelli-Cohen MP
PloS one 2018;13(3):e0194782
PloS one 2018;13(3):e0194782
Iron accumulation in senescent cells is coupled with impaired ferritinophagy and inhibition of ferroptosis.
Masaldan S, Clatworthy SAS, Gamell C, Meggyesy PM, Rigopoulos AT, Haupt S, Haupt Y, Denoyer D, Adlard PA, Bush AI, Cater MA
Redox biology 2018 Apr;14:100-115
Redox biology 2018 Apr;14:100-115
Targeted Metabolomics Reveals Abnormal Hepatic Energy Metabolism by Depletion of β-Carotene Oxygenase 2 in Mice.
Wu L, Guo X, Lyu Y, Clarke SL, Lucas EA, Smith BJ, Hildebrand D, Wang W, Medeiros DM, Shen X, Lin D
Scientific reports 2017 Nov 7;7(1):14624
Scientific reports 2017 Nov 7;7(1):14624
Regulation of iron homeostasis by the p53-ISCU pathway.
Funauchi Y, Tanikawa C, Yi Lo PH, Mori J, Daigo Y, Takano A, Miyagi Y, Okawa A, Nakamura Y, Matsuda K
Scientific reports 2015 Nov 12;5:16497
Scientific reports 2015 Nov 12;5:16497
The diabetes drug target MitoNEET governs a novel trafficking pathway to rebuild an Fe-S cluster into cytosolic aconitase/iron regulatory protein 1.
Ferecatu I, Gonçalves S, Golinelli-Cohen MP, Clémancey M, Martelli A, Riquier S, Guittet E, Latour JM, Puccio H, Drapier JC, Lescop E, Bouton C
The Journal of biological chemistry 2014 Oct 10;289(41):28070-86
The Journal of biological chemistry 2014 Oct 10;289(41):28070-86
Cochaperone binding to LYR motifs confers specificity of iron sulfur cluster delivery.
Maio N, Singh A, Uhrigshardt H, Saxena N, Tong WH, Rouault TA
Cell metabolism 2014 Mar 4;19(3):445-57
Cell metabolism 2014 Mar 4;19(3):445-57
MicroRNA-210 decreases heme levels by targeting ferrochelatase in cardiomyocytes.
Qiao A, Khechaduri A, Kannan Mutharasan R, Wu R, Nagpal V, Ardehali H
Journal of the American Heart Association 2013 Apr 22;2(2):e000121
Journal of the American Heart Association 2013 Apr 22;2(2):e000121
MMS19 assembles iron-sulfur proteins required for DNA metabolism and genomic integrity.
Stehling O, Vashisht AA, Mascarenhas J, Jonsson ZO, Sharma T, Netz DJ, Pierik AJ, Wohlschlegel JA, Lill R
Science (New York, N.Y.) 2012 Jul 13;337(6091):195-9
Science (New York, N.Y.) 2012 Jul 13;337(6091):195-9
No comments: Submit comment
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- mouse heart tissue were subjected to SDS PAGE followed by western blot with 14812-1-AP(ISCU antibody) at dilution of 1:500
- Sample type
- tissue
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of MCF-7 cells, using ISCU antibody 14812-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
- Sample type
- cell line
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- IP Result of anti-ISCU (IP:14812-1-AP, 3ug; Detection:14812-1-AP 1:500) with mouse heart tissue lysate 4000ug.
- Sample type
- tissue
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunohistochemical of paraffin-embedded human lung cancer using 14812-1-AP(ISCU antibody) at dilution of 1:50 (under 40x lens)
- Sample type
- tissue
