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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Immunocytochemistry [2]
- Immunohistochemistry [2]
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- Product number
- PA5-30470 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ISCU Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: U87-MG, SK-N-SH, IMR32, SK-N-AS, ISCU-transfected 293T. Predicted reactivity: Mouse (97%), Rat (97%), Drosophila (84%), Rhesus Monkey (100%), Bovine (99%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.66 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of ISCU was performed in HeLa cells fixed in ice-cold MeOH for 5 min. Green: ISCU Polyclonal Antibody (Product # PA5-30470) diluted at 1:500. Blue: Hoechst 33342 staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of ISCU was performed in HeLa cells fixed in ice-cold MeOH for 5 min. Green: ISCU Polyclonal Antibody (Product # PA5-30470) diluted at 1:500. Blue: Hoechst 33342 staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded A549 xenograft, using ISCU (Product # PA5-30470) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- ISCU Polyclonal Antibody detects ISCU protein at cytosol on mouse muscle by immunohistochemical analysis. Sample: Paraffin-embedded mouse muscle. ISCU Polyclonal Antibody (Product # PA5-30470) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
