Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- AP6165a - Provider product page
- Provider
- Abcepta
- Proper citation
- Abgent Cat#AP6165A, RRID:AB_2138126
- Product name
- MAGEA3 Antibody (C-term)
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Purified Rabbit Polyclonal Antibody (Pab)
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 400 µl
- Concentration
- 2 mg/ml
- Storage
- Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Submitted references MAGE-C1/CT7 is the dominant cancer-testis antigen targeted by humoral immune responses in patients with multiple myeloma.
MAD-CT-2 identified as a novel melanoma cancer-testis antigen using phage immunoblot analysis.
The cancer/testis antigen melanoma-associated antigen-A3/A6 is a novel target of fibroblast growth factor receptor 2-IIIb through histone H3 modifications in thyroid cancer.
Curioni-Fontecedro A, Knights AJ, Tinguely M, Nuber N, Schneider C, Thomson CW, von Boehmer L, Bossart W, Pahlich S, Gehring H, Moch H, Renner C, Knuth A, Zippelius A
Leukemia 2008 Aug;22(8):1646-8
Leukemia 2008 Aug;22(8):1646-8
MAD-CT-2 identified as a novel melanoma cancer-testis antigen using phage immunoblot analysis.
Dubovsky JA, Albertini MR, McNeel DG
Journal of immunotherapy (Hagerstown, Md. : 1997) 2007 Oct;30(7):675-83
Journal of immunotherapy (Hagerstown, Md. : 1997) 2007 Oct;30(7):675-83
The cancer/testis antigen melanoma-associated antigen-A3/A6 is a novel target of fibroblast growth factor receptor 2-IIIb through histone H3 modifications in thyroid cancer.
Kondo T, Zhu X, Asa SL, Ezzat S
Clinical cancer research : an official journal of the American Association for Cancer Research 2007 Aug 15;13(16):4713-20
Clinical cancer research : an official journal of the American Association for Cancer Research 2007 Aug 15;13(16):4713-20
No comments: Submit comment
Supportive validation
- Submitted by
- Abcepta (provider)
- Main image
- Experimental details
- The anti-MAGEA3 (C-term) Antibody (Cat.#AP6165a) is used in Western blot to detect MAGEA3 in Jurkat lysate.
- Primary Ab dilution
- 1:1000
Supportive validation
- Submitted by
- Abcepta (provider)
- Main image
- Experimental details
- MAGEA3 Antibody (C-term) (Cat. #AP6165a)immunohistochemistry analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of MAGEA3 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
- Primary Ab dilution
- 1:10~50
Supportive validation
- Submitted by
- Abcepta (provider)
- Main image
- Experimental details
- MAGEA3 Antibody (C-term) (Cat. #AP6165a) flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
- Primary Ab dilution
- 1:10~50
